| 摘要: |
| 以 20 日龄中华鳖稚鳖为实验动物, 采用静水停食实验法, 在水温(27.4±1.3)℃、pH 7.1 条件下, 进行了注射鳖源嗜水气单胞菌ZHYYZ-1 引发中华鳖稚鳖感染和致死的定量实验研究。结果表明: (1) 该菌为高致病性菌株, 异常排泄是该菌致稚鳖表露临床感染症状的重要标志; (2) 注射该菌引起稚鳖感染与致死具明显的剂量—时间效应, 随注射菌量递增依次出现不表露临床感染症状、表露临床感染症状而不出现死亡和大量死亡三种情形, 稚鳖表露临床感染和出现死亡的时间均随注射菌量增大而明显缩短, 绝大多数稚鳖表露临床感染和出现死亡的时限分别为2h 和48h; (3) 濒死稚鳖体内该菌的底限含量为3.3×106 CFU/g, 稚鳖体内菌注射含量可控安全范围为(1.2—8.8)× 103CFU/g。
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| 关键词: 嗜水气单胞菌 ZHYYZ-1, 感染率, 死亡率, 中华鳖稚鳖 |
| DOI：10.11693/hyhz201004009009 |
| 分类号: |
| 基金项目:宁波市农业科技重大攻关项目, 2007C10025 号。 |
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| QUANTITATIVE ANALYSIS OF INFECTION AND LETHAL EFFECTS ON JUVENILE TRIONYX SINENSIS BY INJECTING PATHOGENICENIC AEROMONAS HYDROPHILA ZHYYZ-1 |
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WANG Zhi-Zheng1, SHEN Tu-Yan1,2, ZHAO Bei-Bei1, RUAN Bo1
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1.Zhejiang Ocean University;2.Fishery Technology Extension Center of Yuyao
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| Abstract: |
| Quantitative analysis of infection and lethal effects on juvenile Trionyx sinensis was studied in the standing water without feeding at (27.4±1.3)℃ by injecting pathogenicenic Aeromonas hydrophila named ZHYYZ-1, which was isolated from the same species of turtle. It was found that: (1) the bacteria strain isolated from the infected turtle was a pathogenic, leading to abnormal excretion; (2) the infection and lethal effects were dose- and time-dependent. There were 3 types of symptoms after the injection, depending on the increases of the amount of bacterial injected: no clinical infection, clinical infection but caused no death, clinical infection caused mass death; (3) the times when the young turtle showed clinical infection symptoms (2h) and death (48h) had a negative correlation with the increases of the amount of bacterial injected; (4) the lowest amount of pathogenic bacterial in impending death turtle was 3.3×106 CFU/g, and the safe range of bacterial number in a turtle was between 1.2×103 CFU/g to 8.8×103 CFU/g.
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| Key words: Aeromonas hydrophila ZHYYZ-1, Infection rate, Mortality, Juvenile Trionyx sinensis |
