| 摘要: |
| 采用线粒体DNA 16S rRNA基因序列测定方法, 对中国沿海 3目 8科的14种腹足类的系统演化关系进行了比较分析。结果显示, 共测定了 102 个个体, 获得线粒体 DNA 16S rRNA 基因片段507bp 的同源碱基序列; 用最大简约法检测到保守位点 87 个, 可变位点 409 个, 简约信息位点 254个; 计算了碱基替换/颠换率的距离(R)和基于碱基替换+颠换率的距离(D), 红螺属内的脉红螺和红螺 R 值为 0.9367, 大于 0.5, 属于种间标志值; 采用 Kimura 2-parameter model 构建了邻接法(NJ)、最小进化法(ME)和最大似然法(MP)等 3 种系统树, 脉红螺和红螺各自均为单系群(支持率= 92—100), 同时表明它们在检测的 14 种腹足类中属于相当进化种类。 分析结果从线粒体基因序列的层次支持脉红螺与红螺属于不同种类的现代分类方法。
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| 关键词: 脉红螺, 红螺, 16S rRNA 基因, 系统发生 |
| DOI：10.11693/hyhz201005013013 |
| 分类号: |
| 基金项目:国家“863”计划“中国近海贝类资源的采集、保存与信息化技术”, 2007AA09Z433 号。 |
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| TAXONOMIC RELATIONSHIP BETWEEN RAPANA VENOSA AND RAPANA BEZOAR BASED ON 16S rRNA GENE SEQUENCES |
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YANG Jian-Min,ZHENG Xiao-Dong,LI Qi,WANG Ru-Cai,WANG Wei-Jun,SUN Guo-Hua,ZHANG Yu
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1.Shandong Marine Fisheries Research Institute;2.Fisheries College, Ocean University of China
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| Abstract: |
| 14 marine gastropods, belonging to 3 orders and 8 families, were phylogenically analyzed based on partial sequences of the mitochondrial 16S rRNA gene. As a result, a homologous nucleotide sequence with 507 base pair was obtained from 102 individuals; 87 conserved sites, 409 variable sites and 254 parsim-info sites were discovered by the method of Maximum Parsimony; D (transition+transversion)-distance and R (transition/transversion)-distance were calculated, and the value R was 0.9367 (>0.5) between Rapana venosa and R. bezoar, which showed different species; Neighbor-Joining, Minimum Evolution and Maximum Parsimony trees were constructed based on the Kimura 2-parameter model, which supported that R. venosa and R. bezoar belonged to homogroup (support values: 92—100); and R. venosa and R. bezoar were also much higher than the other 12 gastropods in evolutionary level. In a word, R. venosa and R. bezoar belonged to different species based on mtDNA 16S rRNA gene sequences data, which was in of accord with modern taxonomy.
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| Key words: Rapana venosa, Rapana bezoar, 16S rRNA gene, Phylogeny |
