| 摘要: |
| 根据鮋形目鱼类 Cytb和 COⅡ上下游保守序列各自设计引物, 对日本鬼鮋的线粒体 DNA进行 PCR 扩增, 克隆并测定了 Cytb和 COⅡ因的全序列, COⅡ基因为 691bp, Cytb为 1141bp。从GenBank 下载 28 种鮋形目及外群鱼类的 Cytb 部分或全序列和 10 种鮋形目及外群鱼类的 COⅡ部分或全序列, 然后进行比对分析, 用 NJ 法和 ML 法构建分子系统发育树。系统发育树结果显示: 线粒体 Cytb和 COⅡ基因序列构建的分子系统树, 与传统分类方法的结果基本一致; 毒鮋科的日本鬼鮋与鮋科的关系最近; 在飞角鱼科(Dactylopteridae)是否属于鮋形目还是单独成豹鲂 目的问题上, 本研究倾向支持飞角鱼科独立归成豹鲂鮋目(Dactylopteriformes)。 日本鬼鮋的 COⅡ与昆虫类的不同, 形目的 COⅡ基因比 Cytb基因保守, 即 COⅡ基因比 Cytb基因进化慢。COⅡ基因进化树相应的节点上数值稍高。
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| 关键词: 日本鬼鮋, Cytb, COⅡ, 系统发育分析 |
| DOI：10.11693/hyhz201101007007 |
| 分类号: |
| 基金项目:长江学者和创新团队发展计划项目, IRT0734 号 |
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| CLONING OF INIMICUS JAPONICUS MITOCHONDRIAL Cyt b AND COⅡ GENE AND THEIR PHYLOGENETIC ANALYSIS IN SCORPAENIFORMES |
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DONG Xu-Hui, LI Ming-Yun, CHEN Jiong, SHI Yu-Hong
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Faculty of Life Science and Biotechnology, Ningbo University
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| Abstract: |
| The entire mitochondrial Cyt b and COⅡsequence of Inimicus japonicus, 691bp for COⅡ and 1141bp for Cyt b, were cloned, whose primers was designed by the upstream and downstream sequences of the mitochondrial Cyt b and COⅡ conserved sequences in Scorpaeniformes. They were compared, respectively, with the mitochondrial Cyt b sequences of 28 other fishes in Scorpaeniformes and the mitochondrial COⅡsequences of 10 other fishes in Scorpaeniformes, based on the molecular phylogenetic trees built by the NJ and ML method. Phylogenetic tree showed that: phylogenetic trees constructed by mitochondrial Cyt b and COⅡsequences were consistent with traditional classification methods; the relations of I. japonicus (Synanceiidae) and Scorpaenidae were mostly close; and Dactylopteridae to build up Dactylopteriformes was more reasonable than to be Scorpaenoidei in this paper. Different from the mitochondrial COⅡ in the insects, the mitochondrial COⅡ gene was more conservative than the mitochondrial Cyt b gene in Scorpaeniformes. Therefore, the value of the nodes in the phylogenetic tree of the mitochondrial COⅡ gene was corresponding slightly higher.
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| Key words: Inimicus japonicus, Cyt b, COⅡ, Phylogenetic analysis |
