| 摘要: |
| 应用 AFLP 技术对文蛤红壳(RS)、黑斑(BS)、细纹(TC)、暗纹(DF)4 个壳色花纹品系进行遗传差异分析。利用 15 对 AFLP 引物组合对 4 品系共 128 个个体进行了 PCR 扩增和电泳检测, 共得到 789 个位点, 总多态位点比例 90.75%。 Nei’s 基因多样性和 Shannon’s 信息指数显示, 4 个品系的遗传多样性大小依次为 BS 品系>RS 品系>DF 品系>TC 品系。 AMOVA 分析表明, 79.45%的变异来自品系内, 品系间的变异只占 20.55% |
| 关键词: 文蛤, 壳色花纹, AFLP, 遗传差异 |
| DOI：10.11693/hyhz201103006006 |
| 分类号: |
| 基金项目:国家自然科学基金项目, 30972255 号; 浙江省自然科学基金项目, Y3110080 号; 温州市重大科技项目, S20080019 号 |
|
| GENETIC VARIATION ANALYSIS OF FOUR STRAINS OF MERETRIX MERETRIX THAT HAVE DIFFERENT SHELL COLORS AND DECORATIVE PATTERNS |
|
ZHU Dong-Li1,2, LIN Zhi-Hua1, DONG Ying-Hui1, YAO Han-Han3
|
|
1.College of Biological and Environmental Sciences, Zhejiang Wanli University;2.College of Fisheries, Ocean University of China;3.College of Fisheries and Life Science, Shanghai Ocean University
|
| Abstract: |
| Genetic variations of four strains of Meretrix meretrix, i.e., thin checkered (TC), black spot (BS), dark fringe (DF) and red shell (RS), were examined using amplified fragment length polymorphism (AFLP). 15 AFLP primer combinations were applied to analyze the genetic diversities and relationships among these strains. 29 strain-specific bands were found in overall 789 detectable bands. These strain-specific bands may be used to distinguish these four strains. The overall percentage of the polymorphic loci of these four strains was 90.75% and the percentage of the polymorphic loci ranged from 61.34% to 75.16%. Nei’s genetic diversity analysis and Shannon’s information index showed that the levels of genetic diversity were in the order of BS > RS > DF > TC. AMOVA analysis indicated that the average fixation index (Fst) was 0.2055%, with 79.45% of variance from within strains and 20.55% of variance from among strains. The results of a cluster analysis indicated that the TC strain had the highest genetic variation, the BS strain ranked the second, and genetic variations between the DF and RS strain were the lowest. One AFLP marker only existed in the TC strain and the frequency was 100%. These results suggested that the unique band of TC strains could be a molecular marker which can be used for strain identification purposes. |
| Key words: Meretrix meretrix, Shell color and decorative pattern, AFLP, Genetic variation |
