| 摘要: |
| 采用 3×3 因子方法, 进行了饲料中不同蛋白质和脂肪水平对克氏螯虾[均重(7.03±0.05)g]生长性能、体组成和消化酶活性的影响研究。试验设 3 个蛋白质水平(24%、27%和 30%)和 3 个脂肪水平(4%、7%和 10%), 共 9 组, 每组 4 个重复, 每个重复 10 尾虾, 为期 8 周。结果表明, 蛋白质和脂肪水平对成活率无显著影响(P>0.05), 增重率和特定生长率显著受脂肪水平影响(P<0.05)。10%脂肪组的增重率及特定生长率显著低于 4%和 7%脂肪组(P<0.05)。饲料中蛋白质水平对全虾的干物质、蛋白和灰分含量, 肌肉蛋白和脂肪含量有显著影响(P<0.05)。 饲料中脂肪水平对全虾和肌肉脂肪含量有显著影响(P<0.05)。饲料蛋白水平和脂肪水平分别显著影响肠道及肝胰脏中蛋白酶活性和脂肪酶活性(P<0.05)。综上所述, 建议克氏螯虾成虾前期饲料的蛋白质水平以 27%左右, 脂肪水平以 4%—7%为宜。 |
| 关键词: 饲料蛋白, 饲料脂肪, 生长性能, 体组成, 消化酶, 克氏螯虾 |
| DOI：10.11693/hyhz201104009009 |
| 分类号: |
| 基金项目:江苏省水产三项工程——克氏螯虾主要营养素需求研究及在配合饲料中的应用, PJ2010-56 号; 公益性行业(农业)科研专项——克氏原螯虾产业技术研究与试验示范, 201003070 号 |
|
| EFFECT OF DIFFERENT DIETARY PROTEIN AND LIPID LEVEL ON GROWTH PERFORMANCE, BODY COMPOSITION AND DIGESTIVE ENZYMES ACTIVITIES OF RED SWAMP CRAYFISH PROCAMBARUS CLARKII |
|
XU Wei-Na1, LIU Wen-Bin1, SHEN Mei-Fang2, WANG Ying1, ZHU Jie1, XU Gang1, CHENG Long1
|
|
1.Key Laboratory of Aquatic Animal Nutrition and Feed Science of Jiangsu Province, College of Animal Science and Technology, Nanjing Agricultural University;2.Freshwater Fisheries Research Institute of Jiangsu Province
|
| Abstract: |
| A bifactorial model (3×3) was used to investigates the effect of dietary protein and lipid on growth performance, body composition and digestive enzymes activities of juvenile red swamp crayfish Procambarus clarkii. Nine groups of crayfish with four replicates and 10 crayfish [average weight (7.03±0.05)g] per replicate were fed nine experimental diets containing three protein levels (24%, 27% and 30%) and three lipid levels (4%, 7% and 10%) for 8 weeks. The result showed that survival rate (SR) of crayfish were not affected significantly (P>0.05) by dietary protein and lipid. Weight gain (WG) and specific growth rate (SGR) of crayfish were affected significantly (P<0.05) by dietary lipid. When grouped by lipid levels, the WG and SGR of crayfish fed the diets containing 10% lipid were lower than that of fish fed the diets containing 4% or 7% lipid (P<0.05). Proximate analysis indicated that whole-body dry matter (DM), crude protein (CP) and ash contents or muscle CP and crude lipid (EF) contents were affected significantly (P<0.05) by dietary protein. Whole-body and muscle crude lipid (EF) were affected significantly (P<0.05) by dietary lipid. Intestinal tract and hepatopancreas protease and lipase activities were affected significantly (P<0.05) by dietary protein and lipid, respectively. In conclusion, positive impacts of optimizing protein and lipid component in terms of economic and environmental concerns, diet containing lipid 4%―7% and protein 27% with protein was beneficial consequences of further refinement of commercial juvenile crayfish production feed. |
| Key words: Dietary protein, Dietary lipid, Growth performance, Body composition, Digestive enzymes, Procambarus clarkii |
