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*青岛文昌鱼(Branchiostoma belcheri tsingtauense)染色体 G 类带型研究*
牟洪善^1,2, 王昌留¹, 李金萍³
1.鲁东大学生命科学学院;2.东华理工大学学团部;3.东华理工大学信息工程学院

摘要:

以青岛文昌鱼的晚期囊胚和早期原肠胚的胚胎细胞为实验材料, 利用低渗处理、空气干燥法制备染色体玻片标本。在此基础上, 为进一步探索显示青岛文昌鱼染色体 G 带的新方法, 采用片龄、蛋白酶 K 和胰蛋白酶显带技术对青岛文昌鱼的染色体进行了初步研究, 显示了青岛文昌鱼染色体的 G 类带型, 绘制了青岛文昌鱼染色体的 G 类带型模式图, 并对三种 G 类带型作了比较, 进而提出了文昌鱼染色体的 G 带带型与片龄的相互关系。

关键词: 青岛文昌鱼, 染色体带型, G 带, 片龄

DOI：10.11693/hyhz201104021021

分类号:

基金项目:国家“863”项目资助, 2008AA092604 号, 山东省教育厅项目资助, J08LE03 号, 江西省教育厅项目资助, GJJ11490 号

STUDIES ON THE G-TYPE CHROMOSOMAL PATTERNS OF AMPHIOXUS BRANCHIOSTOMA BELCHERI TSINGTAUENSE

MU Hong-Shan^1,2, WANG Chang-Liu¹, LI Jin-Ping³

1.College of Life Sciences, Ludong University;2.Students’ Affairs Office, East China Institute of Technology;3.College of Information Engineering, East China Institute of Technology

Abstract:

The chromosomal patterns of amphioxus (Branchiostoma belcheri tsingtauens) were studied using blastula and early gastrula stages of embryos underwent hypotonic treatment and air-drying. New techniques for G-type banding were investigated using trypsin, protease K, and slide aging treatment. The G-type bandings of amphioxus were obtained and the chromosomal profiles were shown. The characteristics of the three G-type bands were compared, the similarities and differences were discussed. In addition, the correlation between the slide aging and G-banding was proposed.

Key words: Amphioxus Branchiostoma belcheri tsingtauense, Chromosome banding, G-banding, Slide aging

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