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盐度和 pH 对合浦珠母贝(Pinctada fucata)耗氧率和排氨率的影响
刘建业,喻达辉,李金碧
1.农业部海水养殖生态与质量控制重点开放实验室 中国水产科学研究院南海水产研究所;2.上海海洋大学水产与生命学院
摘要:
采用实验生态学方法, 对 3 种规格的合浦珠母贝在不同盐度和 pH 下的耗氧率和排氨率进行了研究。结果表明, 盐度对合浦珠母贝的耗氧率和排氨率有极显著影响(P<0.01)。在盐度 21—36的范围内, 耗氧率和排氨率均随盐度的升高而降低, 且单位体重耗氧率、 排氨率随其个体的增大而变小; pH 对合浦珠母贝的耗氧率有极显著影响(P<0.01), 对排氨率有显著影响(P<0.05)。单位体重耗氧率、排氨率也随其个体的增大而变小。在 pH 为 5—9 时, 3 种规格贝耗氧率、排氨率均先升后降, 当pH 为 8 时达到最大值, 分别为 2.467、4.268、7.461mg/(g·h)和 0.406、1.356、2.255mg/(g·h)。随着合浦珠母贝个体的变小, 其 O : N 值有逐渐变小的趋势。在试验盐度 16—36 范围内, O : N 值随着盐度的升高有逐渐变大的趋势。 而 pH 的变化对 O : N 的影响不明显。 在本实验条件下, 总体上 O : N值较低, 表明在盐度和 pH 的胁迫下合浦珠母贝以蛋白质代谢为主。
关键词:  合浦珠母贝, 盐度, pH, 耗氧率, 排氨率
DOI:10.11693/hyhz201104022022
分类号:
基金项目:国家科技支撑计划项目, 2006BAD01A13 号; 广东省科技计划项目, 2006B20201063 号
EFFECTS OF SALINITY AND pH ON OXYGEN CONSUMPTION AND AMMONIA EXCRETION RATES IN PINCTADA FUCATA
LIU Jian-Ye1,2,3, YU Da-Hui1,2, LI Jin-Bi1,2
1.Key Lab. of Mariculture, Ecology and Quality Control, Ministry of Agriculture;2.South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences;3.College of Fisheries and Life Science, Shanghai Ocean University
Abstract:
The effects of salinity and pH on oxygen consumption and ammonia excretion rates in different sizes of Pinctada fucata were studied. The results indicated that the effects of salinity on oxygen consumption rate (Roc) and ammonia excretion rate (Ram) were extremely significant (P<0.01). When salinity changed from 21 to 36, both Roc and Ram decreased. Oxygen consumption rate and ammonia excretion rate decreased with the increase of body sizes. The effect of pH on oxygen consumption rate and ammonia excretion rate were also highly significant, P<0.01 and P<0.05, respectively. In the range of pH 5—9, both oxygen consumption rate and ammonia excretion rate increased at first and reached the highest at pH 8. And then both oxygen consumption rate and ammonia excretion rate decreased with the rise of pH value ranging from 1.041 to 7.461mg/(g·h) and from 1.191 to 2.255mg/(g·h), respectively. At the same time, the O : N ratio decreased with the decrease of body sizes. When salinity changed from 16 to 36, the O : N ratio increased with the increase of body sizes. In the range of pH 5—9, the O : N ratio was not significantly affected. The ratio of O : N was small in total. It indicated that P. fucata increases protein metabolism for surviving under abnormal salinities or pH stresses.
Key words:  Pinctada fucata, Salinity, pH, Oxygen consumption rate, Ammonia excretion rate
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