| 摘要: |
| 构建了多测点激光剥蚀取样(LA)-ICPMS 元素分析-剥蚀点显微结构验证的耳石核区元素指纹分析技术, 以此进行了五个刀鲚群体(辽东湾、渤海湾、黄河口、胶州湾及长江口群体)识别的实证研究。 结果发现, 耳石核区 Sr : Ca 和 Ba : Ca 比值是有效识别各地理群体的元素指纹, 基于二者的各群体判别成功率在 46.2%—92.3%之间, 总体判别成功率为 72.7%, 表现出其较强的群体识别能力。借助于耳石内的放射状标志带结构特征可较准确地识别耳石核区, 保证了激光剥蚀点的精准定位及剥蚀取样的质量和代表性; 对激光剥蚀点的显微结构验证可以消除无效剥蚀点的元素测定数据对元素指纹分析产生的误差; 刀鲚的定居性、溯河性等生活特性降低了各群体混合几率和环境因素对耳石核区微化学组成的影响, 使得耳石核区元素指纹具备高稳定性和特质性。这些研究技术的改进和刀鲚的生理生态特性提高了该技术识别各地理群体的准确性和效率。 |
| 关键词: 耳石, 微化学, 刀鲚, 群体识别, 激光剥蚀, 质谱分析 |
| DOI：10.11693/hyhz201106004004 |
| 分类号: |
| 基金项目:国家自然科学基金项目资助, 40676086 号, 40976084 号, 41121064 号 |
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| MULTIPLE LASER ABLATIONS ON OTOLITH NUCLEI FOR ICPMS TO ELEMENTALLY FINGERPRINT FISH STOCKS: A CASE STUDY |
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DOU Shuo-Zeng1, AMANO Hironori2, YU Xin1,3, CAO Liang1, SHIRAI Kotaro2, OTAKE Tsuguo2, TSUKAMOTO Katsumi4
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1.Key Laboratory of Marine Ecology and Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences;2.International Coastal Research Center, Atmosphere and Ocean Research Institute, The University of Tokyo;3.Graduate School, Chinese Academy of Sciences;4.Atmosphere and Ocean Research Institute, The University of Tokyo
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| Abstract: |
| Elemental fingerprinting on otolith nuclei by laser ablation ICPMS (five laser ablations on the nucleus of each otolith) was conducted to delineate tapertail anchovy (Coilia nasus) stocks from five Chinese estuaries. Results showed that otolith Sr : Ca and Ba : Ca ratios were inter-site distinct enough to be used as natal tags for discriminating among geographical stocks with an overall classification accuracy rate of 75.8% (ranging from 61.5% to 84.6% for each stock by discriminant function analysis). Clear images of the concentric rays from the core outwards on the otolith sections could help us precisely locate the otolith core and conduct the five laser ablations. Subsequent microstructure checking confirmed that all the laser ablations were valid, which reduced the errors in elemental fingerprinting caused by potential invalid ablations and elemental data. The anadromous tapertail anchovy showed high fidelity to natal river for spawning and resided in the local estuary for growth, largely decreasing stock mixing throughout their life time. Meanwhile, the less varied environments in their birth rivers in the spawning season could reduce the ambient effects on the elemental uptake onto the otoliths and thus could produce relatively stable microchemistry composition in the otoliths. These ecological and physiological traits of the tapertail anchovy as well as the improvement of laser ablating method increased the accuracy and efficiency of elemental fingerprinting for stock discrimination obtained in the present study. |
| Key words: Otolith, Microchemistry, Tapertail anchovy Coilia nasus, Stock discrimination, Laser ablation, Mass spectrometry |
