| 摘要: |
| 采用 RT-PCR、RACE、Genome Walking 等方法从中国鲎克隆得到了雌激素相关受体基因的cDNA全长及基因组DNA序列, 命名为ttERR (GenBank登录号为HQ386702), 并采用生物信息学方法对其序列和编码的蛋白质的理化性质、分子系统进化关系等进行预测和推断。结果表明, ttERR 基因 cDNA 全长 2722bp, 包含一个 1413bp 的开放阅读框、626bp 的 5’-UTR 和 683bp 的 3’-UTR, 编码一个由 470 个氨基酸组成的蛋白质。ttERR 基因由 10 个内含子和 11 个外显子组成。系统发育分析表明, ttERR 属于核受体超家族第三亚族。本文为将来深入研究中国鲎 ttERR 的功能奠定了基础。 |
| 关键词: 雌激素相关受体, 中国鲎, 分子克隆, 生物信息学分析 |
| DOI：10.11693/hyhz201106005005 |
| 分类号: |
| 基金项目:国家自然科学基金资助项目, 41076081 号 |
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| CLONING AND ANALYSIS OF ESTROGEN RELATED RECEPTOR GENE FROM TACHYPLEUS TRIDENTATUS |
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HUANG Jing-Ru, LI Wen-Xing, HUANG Hui-Yang, YE Hai-Hui, LI Shao-Jing
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College of Oceanography and Environmental Science, Xiamen University
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| Abstract: |
| In this paper, a homolog of ERRs (designated ttERR) was isolated from the horse crab Tachypleus tridentatus using RT-PCR, RACE and Genomic Walking methods. The nucleotide sequence and deduced amino acid sequence of ttERR were analyzed and characterized by bioinformatics methods. The results showed that the full-length cDNA of ttERR gene is 2722bp, containing a 5′-untranslated region (5′-UTR) of 626bp and a 3′-UTR of 683bp. The open reading frame of 1413bp encodes a 470-amino acid protein. The ttERR gene is composed of ten introns and eleven exons. Phylogram indicated that ttERR belongs to the NR3 group of nuclear receptors subfamily. This study may establish theoretical foundation for further understanding the functions of ttERR. |
| Key words: Estrogen related receptor (ERR), Tachypleus tridentatus, Molecular cloning, Bioinformatic analysis |
