| 摘要: |
| 采用RT-PCR方法克隆了黄鳍鲷两种生长激素受体(Growth hormone receptor, GHR)的cDNA序列, 序列分析表明: GHR1开放阅读框为1935bp, 共编码645个氨基酸, GHR2开放阅读框为1749bp, 共编码 583 个氨基酸, GHR1 与 GHR2 的氨基酸同源性为 36.7%。GHR1 和 GHR2 在分子结构上存在显著差异, GHR1 胞外域有 7 个半胱氨酸残基而 GHR2 只有 6 个; GHR1 胞内域有 9 个酪氨酸残基而GHR2 只有 5 个, 两者的结构差异表明两者可能具有不同的生物学功能。用 Real-time RT-PCR 方法研究了 GHR1 和 GHR2 在各组织的分布情况, 结果表明: GHR1 和 GHR2 在所检测的 10 种组织中均有表达, 其中以肝脏、肌肉、垂体表达量较高, 且在大部分组织中 GHR2 表达量均高于 GHR1。 |
| 关键词: 黄鳍鲷, 生长激素受体, cDNA 克隆, Real-time RT-PCR, 组织表达 |
| DOI：10.11693/hyhz201106013013 |
| 分类号: |
| 基金项目:国家重点基础研究发展计划(973 计划)项目, 2010CB126302 号; 广东省自然科学基金资助项目, 9451601501001986 号; 广东省教育部产学研结合项目, 2010B090400551 号 |
|
| cDNAs CLONING AND TISSUES EXPRESSION OF TWO GROWTH HORMONE RECEPTORS IN YELLOW-FIN BREAM SPARUS LATUS |
|
MA Xi-Lan1,2,3, LENG Ting-Ting1, LIU Qi-Zhi1, ZHOU Li-Bin4, ZHANG Yong1, LIN Hao-Ran1
|
|
1.State Key Laboratory of Biocontrol, Institute of Aquatic Economic Animals and Guangdong Provincial Key Laboratory for Aquatic Economic Animals, School of Life Sciences, Sun Yat-Sen University;2.School of Life Sciences, South China Normal University;3.Departm;4.Department of Life Science, Institute of Biotechnology, Huizhou University
|
| Abstract: |
| Growth hormone (GH) has many important physiological roles in the control of growth, metabolism and reproduction, which is mediated by growth hormone receptor (GHR). In this study, two cDNAs encoding GHR were isolated from the liver of yellow-fin bream Sparus latus. The two cDNAs, one consisting of 1935bp and the other of 1749bp, encoding for putative 645- and 583-amino acid GHR (designated GHR1 and GHR2, respectively), shared 36.7% identity in deduced amino acid sequence. GHR1 and GHR2 showed the conserved structural characteristics of GHR family, including the FGEFS motif, the box1 and box2 regions, extracellular cysteine residues and intracellular tyrosine residues. However, there were differences of structural features between the two receptors as well. GHR2 lacked one pair of extracellular cysteines and 4 intracellular tyrosine residues which were conservative in GHR1. The structural discrepancies thus undoubtedly indicated the distinct biological functions of GHR1 and GHR2. Real-time RT-PCR analysis showed that both GHR1 and GHR2 mRNAs were presented in all tissues tested and expressed extremely highly in the liver. In most tissues, GHR2 expressed significantly higher than GHR1. The expression distinction of GHR1 and GHR2 in pituitary and peripheral tissues like liver, muscle, spleen, kidney and gonad indicated that the two receptors may have different biological functions. |
| Key words: Yellow-fin bream Sparus latus, Growth hormone receptor, cDAN clone, Real-time PCR, Tissue expression |
