| 摘要: |
| 采用扫描电镜、光谱分析和元素分析法, 进行了胃蛋白酶水解法、高速离心法和酸水解法精制的鱿鱼墨黑色素颗粒形态、紫外可见光谱、红外光谱及金属元素含量的研究。结果表明, 酸水解法破坏了鱿鱼墨黑色素的颗粒结构; 三种不同精制方法所得的鱿鱼墨黑色素在 220nm 附近均有强吸收。胃蛋白酶水解和酸水解法有效切除黑色素的氨基基团, 但酸水解法下鱿鱼墨黑色素吲哚类化合物的特征峰由 1399.64cm-1移至 1307.88cm-1; 高速离心法精制的黑色素则在 2925.02cm-1和2854.09cm-1有新吸收峰。不同精制方法对鱿鱼墨黑色素自身的不同金属元素含量具有降低和富集效应。 |
| 关键词: 鱿鱼墨, 黑色素精制, 颗粒形态, 光谱特征, 金属元素分析 |
| DOI：10.11693/hyhz201106014014 |
| 分类号: |
| 基金项目:浙江省水产品加工产业创新团队项目, 2011R09031-08 号; 浙江省自然科学基金项目, Y3110153 号; 浙江省优先主题重点项目, 2011C13027-2 号 |
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| EFFECTS OF DIFFERENT REFINING METHODS ON THE MICRO-MORPHOLOGY, SPECTRA PROPERTIES AND METAL ELEMENTS OF MELANIN FROM SQUID (OMMASTREPHES BARTRAMI) INK |
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SONG Ru1, LI Ren-Wei2, YU Qun-Di1, DENG Shang-Gui1
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1.School of Food Science and Pharmacy, Zhejiang Ocean University;2.Beijipin Aquactic Product Co. Ltd. of Zhejiang Province
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| Abstract: |
| In this study, pepsin hydrolysis, high speed centrifugation and acidic hydrolysis were used to refine the melanin from squid (Ommastrephes bartrami) ink, and the characteristics of surface morphology, optical spectra and metal elements of these refined squid melanin were investigated. Results of scanning electronic microscope (SEM) showed that acidic hydrolysis damaged the melanin’s original morphology. These refined squid melanin had maximum absorbance around 220nm. Fourier transform infra-red (FTIR) spectra revealed that pepsin hydrolysis and acidic hydrolysis cut off amino groups from squid melanin. However, the characteristic peak of indole group of melanin shifted from 1399.64cm-1 to 1307.88cm-1 after acidic hydrolysis. High speed centrifugation for squid melanin resulted in forming new peaks at 2925.02cm-1 and 2854.09cm-1, respectively. Moreover, the different refining methods lead to the effects of decrease or enrichment on the contents of metal elements in squid melanin. |
| Key words: Squid ink, Refining melanin, Granular morphology, Spectra properties, Metal element analysis |
