| 摘要: |
| 采用显微镜和连续切片技术, 观察了大菱鲆胚胎发育阶段的形态学和组织学特征。结果表明, 大菱鲆胚胎发育主要经历 6 个时期; 在 14℃, 胚体经 108h 即可孵化出膜。胚胎在 64 细胞期出现纬裂, 分裂球分化为外层的包被层和内部的深层细胞; 多细胞期形成卵黄合胞体层; 低囊胚期形成囊胚腔。受精后 26h 30min 胚盾出现。胚盘下包 65%时, 头突出现; 下包 90%时, 神经索和体节形成; 下包完全时, 脊索原基形成。 受精后59h 35min, 克氏囊出现; 72h消化管出现; 74h前肾小管形成; 82h 脊索部分细胞发生“真空化”; 89h 克氏囊退化消失, 胚胎消化管的后端出现纤毛; 94h 胚体的心跳开始。初孵仔鱼鳍褶上形成特有的色素丛。 |
| 关键词: 大菱鲆, 胚胎, 发育, 形态学, 组织学 |
| DOI：10.11693/hyhz201106015015 |
| 分类号: |
| 基金项目:现代农业产业技术体系建设专项资金资助项目, nycytx-50 号; 国家高技术研究发展计划(863)项目, 2006AA10A404 号 |
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| MORPHOLOGICAL AND HISTOLOGICAL DEVELOPMENT OF THE EMBRYO OF TURBOT SCOPHTHALMUS MAXIMUS |
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TONG Xue-Hong1,2,3, MA Dao-Yuan1, XU Shi-Hong1, LIU Qing-Hua1, ZHAO Chun-Yan1,3, XIAO Zhi-Zhong1, XIAO Yong-Shuang1, LI Jun1
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1.Institute of Oceanology, Chinese Academy of Sciences;2.College of Life Science and Technology, Yancheng Teachers University;3.Graduate School, Chinese Academy of Sciences
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| Abstract: |
| Morphological and histological characteristics of embryonic development of turbot were studied. Embryonic development of turbot was divided into six main stages. Hatching occurred 108 hours post-fertilization (hpf) at 14℃. The horizontal cleavage firstly appeared at 64-cell stage, when blastomere differentiated into the enveloping layer on the periphery and the deep cells in the center. Yolk syncytial layer and blastocoel were formed at multiple-cell stage and low stage, respectively. At 26.5hpf, embryonic shield occurred. Head anlage appeared at 65%-epiboly stage, neural rod and somite formed at 90%-epiboly stage, and notochord primordium formed at complete epiboly stage. Kupffer’s vesicle occurred at 59h 35min hpf, digestive tube occurred at 72 hpf, pronephric tubule occurred at 74 hpf, and the vacuolization of notochord occurred at 82 hpf. Kupffer’s vesicle degenerated at 89 hpf, and the posterior digestive tract of embryo was ciliated. At 94 hpf, the heart began to beat. The specific pigment cluster appeared in the fin fold of the newly hatched larva. |
| Key words: Scophthalmus maximus, Embryo, Development, Morphology, Histology |
