| 摘要: |
| 采用海水盐度由25突降至21、17和13胁迫大黄鱼(Pseudosciaena crocea)的方法, 研究了48h内血清生理生化和鳃丝Na+/K+-ATP酶活性的变化。结果表明, 实验过程中三个盐度突降组的血清Na+、Ca2+离子浓度均未发生显著变化(P0.05), 血清K+浓度均显著升高(P<0.05), 且升高幅度与盐度突降幅度呈正相关, 最大值达14.03mmol/L(盐度13组, 48h); 血清Cl?浓度在盐度21组未发生显著变化(P>0.05), 17和13组则在48h时显著降低(P<0.05); 三个盐度突降组的血清酶ALT、AST、LDH、CK-MB活性均显著高于对照组(P<0.05), 随胁迫时间的延长呈先升后降的趋势, 且变化幅度均与盐度突降幅度呈正相关; 三个盐度突降组的鳃丝Na+/K+-ATP 酶活力均呈先升后降的变化趋势, 除盐度21组在12h时高于对照组外, 活力均显著低于对照组(P<0.05), 且降低幅度与盐度突降幅度呈正相关; 到实验15天时, 死亡率随盐度突降幅度增大而升高。 |
| 关键词: 大黄鱼 盐度 血清离子 血清酶 鳃丝 Na+/K+-ATP酶活性 |
| DOI：10.11693/hyhz201302025025 |
| 分类号: |
| 基金项目:国家高技术研究发展计划(863)项目, 2012AA100803 号; 宁波市重大项目资助, 2008C10022 号; 长江学者和创新团队发展计划项目资助, IRT0734 号 |
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| EFFECTS OF ABRUPT SALINITY DECREASE ON SERUM PHYSIOLOGY, BIOCHEMISTRY AND GILL Na+/K+-ATPase ACTIVITY OF THE LARGE YELLOW CROAKER PSEUDOSCIAENA CROCEA |
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WANG Tao, MIAO Liang, LI Ming-Yun, HU Mou, CHEN Jiong, SHI Yu-Hong, LU Xin-Jiang
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School of Marine Sciences, Ministry of Education Key Laboratory of Applied Marine Biotechnology, Ningbo University
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| Abstract: |
| The effects of abrupt salinity decrease at varying degrees (from 25 to 21, 17 and 13) on serum physiology, biochemistry and gill Na+/K+-ATPase activity in large yellow croaker Pseudosciaena croceaduring 48h were studied. The results showed that serum Na+ and Ca2+ had no significant changes (P>0.05) during the whole experiments, the serum K+ increased significantly (P<0.05) and the increasing amplitude was positive correlated to salinity decreased degree (the maximum was 14.03mmol/L in 13 group at 48h), and the serum Cl- decreased significantly (P<0.05) at 48h in 17 and 13 groups. In all the three abrupt salinity decreased groups, the enzyme activities of ALT, AST, LDH and CK-MB increased at first and then decreased, they were all significantly higher (P<0.05) than the control group and the increasing amplitudes were positive correlated to salinity decreased degree. The gill Na+/K+-ATPase activities increased at first and then decreased in all the three abrupt salinity decreased groups, they were all significantly lower (P<0.05) than the control group (the exception was in 21 group at 12h) and the decreasing amplitudes were positive correlated to salinity decreased degree. The mortalities at 15d showed a positive correlation to the salinity decreased degree. |
| Key words: Pseudosciaena crocea salinity serum ions serum enzyme gill Na+/K+-ATPase |
