| 摘要: |
| 采用线粒体(mtDNA)DNA COI 和Cyt b 基因片段序列分析方法, 进行了中国近海条石鲷(Oplegnathus fasciatus)舟山和胶南群体遗传多样性及其遗传结构现状研究。结果表明, 基于mtDNA COI分子标记揭示条石鲷舟山群体遗传多样性水平(h= 0.814±0.060, π= 0.009±0.005, k= 5.653±2.789)显著高于胶南群体(h= 0.742±0.116, π= 0.003±0.002, k= 1.970±1.196); MST分析、NJ系统分析和核苷酸不配对分布分析结果皆显示两群体内存在三个显著分化的单倍型类群, 支系间的遗传距离为0.018—0.025; 遗传分化指数结果(Fst= 0.331, ?P= 0.000)和确切P检验结果显示(P= 0.000)两群体间存在显著遗传分化。基于mtDNA Cyt b分子标记显示条石鲷群体遗传多样性总体上呈现较低水平(h= 0.874±0.023, π= 0.006± 0.003, k= 2.761±1.492), 舟山群体核苷酸多样度水平显著高于胶南群体; NJ系统发育和群体遗传分化研究结果显示, 条石鲷群体间未检测到显著的遗传分化。 |
| 关键词: 条石鲷, 群体, 遗传多样性, mtDNA COI 和Cyt b基因, 遗传分化 |
| DOI：10.11693/hyhz201302026026 |
| 分类号: |
| 基金项目:“十二五”农村领域国家科技计划课题, 2012AA10A413-1 号; 中国科学院创新前沿项目, KSCX2-YW-N-47-08 号; 农业部东海与远洋渔业资源开发利用重点实验室开放课题, K201204 号; 海洋渔业科学与技术浙江省重中之重学科开放课题, 20110208号; 山东省优秀中青年科学家科研奖励基金课题, BS2012HZ025 号; 2012 年留学回国人员科技择优资助经费项目, 2013.01—2014.12。 |
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| POPULATION GENETIC DIVERSITY OF ROCK BREAM OPLEGNATHUS FASCIATUS BASED ON mtDNA GENOME SEQUENCE IN CHINESE COAST |
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XIAO Yong-Shuang1,2, CHENG Qi-Qun3, REN Gui-Jing3, LI Jun2, MA Dao-Yuan2, XU Shi-Hong2, LIU Qing-Hua2, XIAO Zhi-Zhong2, HAN Zhi-Qiang1
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1.Zhejiang Ocean University;2.Institute of Oceanology, Chinese Academy of Sciences;3.East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences
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| Abstract: |
| Partial nucleotide sequences of mitochondrial DNA COI and Cyt b genes were sequenced and analyzed to estimate population genetic diversity and genetic structure of rock bream (Oplegnathus fasciatus) in Zhoushan and Jiaonan populations. The results show that the haplotype diversity and nucleotide diversity of Zhoushan population (h= 0.814± 0.060, π = 0.009±0.005) were significantly higher than those of Jiaonan population (h= 0.742±0.116, π= 0.003±0.002) in mtDNA COI molecular marker. Three genealogical clades were checked in the O. fasciatus populations in NJ, MST and Mismatch distribution analyses of mtDNA COI gene sequence, and the genetic distances among the clades ranged from 0.018—0.025. No significant genetic differentiations existed between Zhoushan and Jiaonan populations in the species based on the analyses of NJ and the pairwise fixation index Fst of mtDNA Cyt b gene. |
| Key words: Oplegnathus fasciatus populations genetic diversity mitochondrial DNA COI and Cyt b genes genetic differentiation |
