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| 大菱鲆四倍体的人工诱导研究 |
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吴志昊,尤锋,宋宗诚,胡金伟,王丽娟,谭训刚,李军
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中国科学院海洋研究所 实验海洋生物学重点实验室,中国科学院海洋研究所 实验海洋生物学重点实验室,威海市环翠区水产研究所,中国科学院海洋研究所 实验海洋生物学重点实验室; 中国科学院大学,中国科学院海洋研究所 实验海洋生物学重点实验室,中国科学院海洋研究所 实验海洋生物学重点实验室,中国科学院海洋研究所
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| 摘要: |
| 大菱鲆(Scophthalmus maximus)为我国北方沿海主要的海水经济品种, 其四倍体的获得对种质改良和三倍体的规模化生产具有重要的价值。到目前为止, 有关大菱鲆四倍体的人工诱导尚未见报道。本文采用静水压抑制受精卵卵裂的方法, 进行了四倍体诱导条件的摸索。结果显示, 在14.8-15.5°C培育时, 在卵裂前15min 用67.5MPa 处理6min 可获得较好的诱导效果, 其孵化率最高可达72%, 染色体制片计数法计算其原肠胚期诱导率可达70%, 初孵仔鱼流仪检测诱导率最高为73%。诱导组胚胎发育与对照组在形态学上基本一致, 仅发育孵化时间比对照组略有滞后。 |
| 关键词: 大菱鲆 四倍体 人工诱导 静水压 |
| DOI：10.11693/hyhz20130400028 |
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| 基金项目:国家863 计划项目, 2012AA10A402 号; 公益性行业(农业)科研专项经费项目, 200903046 号;国家自然科学基金面上项目,41276171 号 |
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| INDUCTION OF TETRAPLOID IN TURBOT SCOPHTHALMUS MAXIMUS |
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Wu Zhihao,You Feng,Song Zongcheng,Hu Jinwei,Wang Lijuan,Tan Xungang and Li Jun
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Institute of Oceanology, Chinese Academy of Sciences,Institute of Oceanology, Chinese Academy of Sciences,Huancui Fishery Institute of Weihai,Institute of Oceanology, Chinese Academy of Sciences; University of Chinese Academy of Sciences,Institute of Oceanology, Chinese Academy of Sciences,Institute of Oceanology, Chinese Academy of Sciences,Institute of Oceanology, Chinese Academy of Sciences
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| Abstract: |
| Turbot Scophthalmus maximus is an important cultured marine fish in North China. The induction of tetraploid turbot is important in germplasm enhancement for large-scale applications of triploid. However, there is almost no report on artificial induction of tetraploid turbot. In this study, the tetraploid turbot was induced by hydrostatic pressure shock to prevent embryo cleavage. The results show that the optimal initiation time for pressure shock was 15 min before the first cleavage at 14.8-15.5°C. The optimal treatment pressure and treatment duration were 67.5MPa and 6 min. The hatching rate and tetraploid rate could reach 72% and 70%, respectively. This paper provides basic data for production of fertile tetraploid adults in turbot. |
| Key words: Turbot Scophthalmus maximus tetraploid artificial induction hydrostatic pressure shock |