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黄颡鱼(Pelteobagrus fulvidraco)MSTN基因SNP位点与体重的相关性分析
陈校辉1, 刘朋朋2, 王明华1, 陈友明1, 钟立强1, 潘建林1
1.江苏省淡水水产研究所;2.江苏省淡水水产研究所，南京师范大学生命科学学院
摘要:
为研究黄颡鱼(Pelteobagrus fulvidraco)MSTN基因的多态性与体重之间的相关性, 对100条六月龄黄颡鱼肌肉生长抑制素基因(MSTN)的全序列进行测序, 获得了8个多态性较高的SNP位点。结果表明, 其中有7个位点分布于非编码区, 1 个位点位于编码区, 但位于编码区的核苷酸突变为同一突变。得出这8个SNP位点中有3个位点的基因型与黄颡鱼的体重有显著的相关性: IJ与体重呈正相关性, 而NN和OP与黄颡鱼体重呈负相关性。在黄颡鱼育种过程中, 可以尝试利用这三个位点进行分子标记辅助选育。
关键词:  黄颡鱼  肌肉生长抑制基因  体重  相关性
DOI：10.11693/hyhz20130400029
分类号:
基金项目:江苏省科技支撑计划项目, BE2010376 号, BE2012469 号; 江苏省农业科技自主创新基金, CX(11)1036 号。
SNP SITES OF MSTN GENE AND ITS ASSOCIATION WITH BODY WEIGHT IN YELLOW CATFISH (PELTEOBAGRUS FULVIDRACO)
CHEN Xiao-Hui1, LIU Peng-Peng1,2, WANG Ming-Hua1, CHEN You-Ming1, ZHONG Li-Qiang1, PAN Jian-Lin1
1.Freshwater Fisheries Research Institute of Jiangsu Province;2.Nanjing Normal University, School of Life Science
Abstract:
To understand the relationship between body weight and SNP sites of myostatin, myostatin of 100 six-month-old yellow catfish Pelteobagrus fulvidracowere sequenced. Eight SNP loci in high polymorphism were tested, of which seven were in uncode area and one in code area. However, the nucleotide mutation that located in code area, was synonymous mutation. Among eight SNP loci, three had genotype distinctly correlated with the weight of P. fulvidracos’: IJ is in positive correlation and NN and OP in negative. Therefore, we could apply the three loci for molecular-marker- assisted breeding programs in P. fulvidracos.
Key words:  yellow catfish Pelteobagrus fulvidraco  MSTN  body weight  association
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