| 摘要: |
| 澳洲鳕鲈（Maccullochella peelii peelii）为澳大利亚国宴用鱼，已引入国内开始工厂化养殖，但人工繁殖还有若干问题未解决。为实现其全人工繁殖，本研究就澳洲鳕鲈精卵同步、精子存活时间延长、选取室内工厂化养殖培育的个体作为亲本进行繁殖等人工繁殖过程中关键步骤进行了研究。结果表明精子样本常温保存6h后精子动性降低，存活时间显著缩短，96h后未发现游动精子。添加2.5%葡萄糖或1.25%葡萄糖+2.25‰氯化钠激活精子动性升高，存活时间得以延长，提示葡萄糖可能能够充当外源性能量来源，显著增强精子活力、提高繁殖效率。实验选取6-8龄室内工厂化养殖澳洲鳕鲈作为繁殖亲本，运用该方法最终获得50万尾鱼苗，证明了用室内工厂化养殖的个体作为亲本进行人工繁殖是可以满足工厂化养殖苗种需求的。 |
| 关键词: 澳洲鳕鲈 人工繁殖 亲本 精子动性 存活时间 葡萄糖 |
| DOI：10.11693/hyhz20191100214 |
| 分类号:S961.2;S965 |
| 基金项目:江苏省三新工程资助项目，Y2017-42号；农业部948资助项目，2016-X27号；江苏农牧科技职业学院大学生创新项目，201912806027Y号。 |
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| ON PRACTICAL TECHNOLOGY FOR FULL ARTIFICIAL PROPAGATION OF MURRAY COD MACCULLOCHELLA PEELII PEELII |
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LIU Mei-Jian1, ZHAO Zi-Ming1, LIU Ming2, CHEN Su-Nan1, ZHENG Xiang-Long1, LIN A-Peng1, GONG Qing-Li2
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1.Jiangsu Ari-animal Husbandry Vocational College, Taizhou 225300, China;2.China Ocean University, Qingdao 266003, China
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| Abstract: |
| As state banquet fish of Australia, Murray cod Maccullochella peelii peelii has been introduced to China for indoor industrial culture. However, a series of problems remain unsettled. To achieve the full artificial propagation, the key steps in the process of artificial reproduction, such as the synchronization of sperm and egg, the prolongation of sperm survival time, and the selection of individuals cultured in factory as parents, were investigated. Results showed that the motility of spermatozoa decreased and the survival time of spermatozoa significantly shortened after 6 hours of normal temperature storage, and no motile spermatozoa was found after 96 hours. The addition of 2.5% glucose or 1.25% glucose plus 2.25‰ sodium chloride increased sperm motility and prolonged sperm survival time, suggesting that glucose may be used as an exogenous energy resource, significantly enhancing sperm motility, and improving reproductive efficiency. In the experiment, 6-8-year-old Murray cods were selected as the broodstocks, and 500 000 fry were finally obtained using this method. It was proved that using the indoor cultured individuals as broodstocks could meet the needs of fry for factory cultivation. |
| Key words: Murray cod Maccullochella peelii peelii artificial propagation broodstocks sperm motility survival time glucose |
