| 摘要: |
| 全球气候和环境变化影响海洋动物的生长发育和繁殖等生命过程,探究环境因子调控发育的分子机制,需要深入了解海洋动物早期发育的生理和分子特征。以实验室驯化的潜在模式动物海洋线虫Litoditis marina为研究对象,对其胚胎期和孵化后发育早期2h、4h和6h的L1幼虫样品进行了转录组测序和分析。结果表明,2h、4h和6h的L1幼虫间差异较小,而三个L1幼虫样品与胚胎期相比,基因表达发生了显著变化。通过KEGG富集分析,发现与胚胎期相比,三个L1幼虫样品的多个通路如核糖体、核糖体生物发生、糖酵解/糖原异生、TCA循环和氧化磷酸化通路相关基因发生了显著上调。另外还发现多个神经递质和神经肽受体基因如dop-和npr-等在L1期转录水平显著上调。与胚胎期相比,L1幼虫的多个DNA复制和修复相关、Notch、Hippo和Hedgehog信号和剪切体等通路相关基因发生了显著下调。发现的L. marina早期发育转录组变化模式与已经发表的陆生模式生物秀丽线虫Caenorhabditis elegans从胚胎期到L1幼虫的转录组变化特征非常相似,但同一上调或下调通路中具体发生表达变化的基因有些不同。另外,L1幼虫显著上调的核糖体生物发生通路相关基因在秀丽线虫中发生了显著下调。进一步通过基因编辑等技术和方法深入研究发育调控关键基因的功能将为海陆近缘线虫间的发育进化机制、海洋线虫对潮间带环境适应以及全球气候变化应答的分子机制研究提供新认知。 |
| 关键词: 海洋线虫 Litoditis marina 早期发育 核糖体 能量 代谢 神经受体 DNA复制和修复 |
| DOI：10.11693/hyhz20220300057 |
| 分类号:Q344+.1 |
| 基金项目:国家重点研发项目,2018YFD0901301号;青岛创业创新领军人才项目,Grant16-8-3-19-zhc号;中国科学院海洋大科学研究中心重点部署项目,2019.11~2022.11。 |
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| TRANSCRIPTOME ANALYSIS OF MARINE NEMATODE LITODITIS MARINA IN EARLY DEVELOPMENTAL STAGES |
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WANG Tong-Tong1,2,3,4, ZHANG Liu-Suo1,2,3
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1.CAS Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China;2.Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266237, China;3.Center for Ocean Mega-Science, Chinese Academy of Sciences, Qingdao 266071, China;4.University of Chinese Academy of Sciences, Beijing 100049, China
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| Abstract: |
| Global climate and environmental changes affect the development, reproduction, and other life processes of marine animals. To explore the molecular mechanism underlying how environmental factors regulate animal development, it is necessary to know the physiological and molecular nature of marine animals in early developmental stages. Transcriptome sequencing of samples taken from the embryonic stage and 2 h, 4 h and 6 h of L1 larvae of Litoditis marina, a potential model animal domesticated in our laboratory for about five years, were analyzed. Results showed small differences in gene expression among L1 larvae at 2 h, 4 h and 6 h, but significant difference from that of the embryonic stage. Multiple pathways such as ribosome, ribosome biogenesis, glycolysis/gluconeogenesis, TCA cycle, and oxidative phosphorylation related genes were significantly up-regulated in the three L1 larval samples compared to that of the embryonic stage, as shown in KEGG enrichment analysis. In addition, several neurotransmitter and neuropeptide receptor genes, such as dop- and npr-, were also significantly increased in L1 larvae. Several genes that are related to DNA replication and repair, Notch, Hippo, and Hedgehog signaling and spliceosome pathways were significantly down-regulated in L1 larvae compared with the embryonic stage. Therefore, the change pattern of the early developmental transcriptome of L. marina is very similar to that of the terrestrial model organism Caenorhabditis elegans. However, the specific genes expressed in the same up-regulated or down-regulated pathway were somewhat different. In addition, genes related to the ribosome biogenesis pathway that were significantly up-regulated in L. marina L1 larvae, were instead significantly decreased in C. elegans L1 larvae, compared to the embryos. In the future, the functions of key candidate genes regulating development through gene editing shall be studied, which will provide new insights into mechanisms underlying evolutionary developmental regulation between marine and terrestrial nematode relatives, the adaptation to the intertidal environments, and how marine animals response and adapt to the global climate change. |
| Key words: marine nematode Litoditis marina early development ribosome energy metabolism neuronal receptors DNA replication and repair |
