| 摘要: |
| 旨在探究养殖水体中添加球形赖氨酸芽孢杆菌对斑马鱼幼鱼生长、抗氧化免疫、肠道菌群与代谢的影响。将初体重为(0.019±0.003) g的270尾斑马鱼随机均分为3组, ZF0组(对照组, 基础饲料)、ZF5组(基础饲料+105CFU/mL球形赖氨酸芽孢杆菌)与ZF7组(基础饲料+107CFU/mL球形赖氨酸芽孢杆菌), 每组3个平行,试验周期为33 d。实验结果表明: (1) 各组间的终末体重、增重率和特定生长率都没有显著性差异(P>0.05)。(2) ZF5和ZF7组的GPT活性显著高于ZF0组(P<0.05), ZF5组AKP的活性显著高于其他组(P<0.05)。(3) ZF7组胰蛋白酶和脂肪酶的活性相对于ZF0和ZF5组显著提高(P<0.05)。(4) ZF7组肠道绒毛高度、V/C都显著高于ZF0与ZF5组(P<0.05); ZF7组的隐窝深度显著低于其他两组(P<0.05)。(5) 从肠道菌群组成来看, 各组间优势菌门均为变形菌门(Proteobacteria), ZF5和ZF7组显著降低了肠道中梭杆菌门(Fusobacteriota)的丰度并显著提高了放线菌门(Actinobacteriota)的丰度(P<0.05)。属水平上, 对照组与试验组的优势菌属为鲸杆菌属(Cetobacterium), 试验组显著减少了邻单胞菌属(Plesiomonas)的丰度同时显著提高了戈登氏菌属(Gordonia)、细杆菌属(Microbacterium)等有益菌属的丰度(P<0.05)。(6)ZF5和ZF7组与ZF0组的肠道代谢物差异明显, 其中Lipoxin a4、Prostaglandin g2和11-dehydrothromboxane b2显著上调(P<0.05), 5'-phosphoribosyl- 5-amino-4-imidazolecarboxamide (aicar)显著下调(P<0.05)。差异代谢物主要富集在ABC转运蛋白、神经活性配体-受体相互作用以及丙氨酸、天冬氨酸和谷氨酸通路中。综上所述,养殖水体中添加球形赖氨酸芽孢杆菌有利于改善斑马鱼幼鱼的肠道消化性能、组织结构和微生物多样性及其代谢产物。 |
| 关键词: 球形赖氨酸芽孢杆菌 斑马鱼幼鱼 生长性能 抗氧化免疫 肠道菌群 代谢 |
| DOI：10.11693/hyhz20230900188 |
| 分类号:Q93-331;Q936;S965 |
| 基金项目:国家自然科学基金项目,47176119号;舟山市科技计划项目,2020C21008号;浙江海洋大学科研启动项目,11105090718号。 |
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| EFFECTS OF LYSINIBACILLUS SPHAERICUS ON GROWTH, INTESTINAL FLORA AND METABOLISM OF JUVENILE ZEBRAFISH (DANIO RERIO) |
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WANG Han-Ying1, ZHANG Xin-Yue2, PENG Huai-Yun2, YANG Jun-Ye2, QIU Hao-Yu2, HUANG Ling2, TAO Chen-Zhi2, WANG Ping2
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1.National Marine Facility Breeding Engineering Technology Research Center, Zhejiang Ocean University, Zhoushan 316022, China;2.College of Marine Science and Technology, Zhejiang Ocean University, Zhoushan 316022, China
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| Abstract: |
The purpose of this study was to investigate the effects of Lysinibacillus sphaericus added to cultured water on the growth, antioxidant immunity, intestinal flora and metabolism of juvenile zebrafish. 270 juvenile zebrafish with an initial body weight of (0.019±0.003) g were randomly divided into 3 groups: ZF0 group (the control group, the basic diet), ZF5 group (the basic diet +105 CFU/mL L. sphaericus) and ZF7 group (the basic diet +107 CFU/mL L. sphaericus). The experiment period was 33 days. The results showed as follows: (1) There were no significant differences in the final body weight, weight gain rate and specific growth rate among all groups (P>0.05). (2) The GPT activity of ZF5 and ZF7 groups was significantly higher than that of ZF0 group (P<0.05), and the AKP activity of ZF5 group was significantly higher than that of other groups (P<0.05). (3) The activities of trypsin and lipase in ZF7 group were significantly increased compared with those in ZF0 and ZF5 groups (P<0.05). (4) Villus height and V/C in ZF7 group were significantly higher than those in ZF0 and ZF5 groups (P<0.05); The crypt depth of ZF7 group was significantly lower than that of the other two groups (P<0.05). (5) In terms of intestinal flora composition, ZF5 and ZF7 groups significantly decreased the abundance of Clostriobacteria and significantly increased the abundance of Actinobacteria. On the genus level, the abundance of Gordonia, Microbacterium and other beneficial bacteria significantly increased in experimental group (P<0.05). (6) Intestinal metabolites of ZF5 and ZF7 groups were significantly different from those of ZF0 group, Lipoxin a4, Prostaglandin g2 and 11-dehydrothromboxane b2 were significantly upregulated (P<0.05) and 5' -Phosphoribosyl-5-amino-4-imidazolecarboxamide (aicar) was significantly downregulated (P<0.05). Differential metabolites were mainly concentrated in ABC transporters, neuroactive ligand-receptor interaction, alanine, aspartate and glutamate metabolism pathways. In conclusion, the addition of L. sphaericus in cultured water can improve the intestinal digestive performance, microbial diversity and metabolites of juvenile zebrafish. |
| Key words: Lysinibacillus sphaericus juvenile zebrafish growth performance antioxidant and immunity intestinal flora metabolism |
