引用本文:	程红艳,陈军辉,张道来,赵恒强,郑立,臧家业,王小如.超声波辅助提取RP-HPLC法测定浒苔中的叶绿素a、b[J].海洋科学,2010,34(2):23-27.

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超声波辅助提取RP-HPLC法测定浒苔中的叶绿素a、b
程红艳¹, 陈军辉¹, 张道来¹, 赵恒强¹, 郑立¹, 臧家业¹, 王小如^2,1
1.国家海洋局第一海洋研究所海洋生态研究中心;2.厦门大学化学化工学院化学系

摘要:

建立了一种反相高效液相色谱法(RP-HPLC)测定浒苔(Enteromorpha prolifera)中叶绿素a和b含量。采用的色谱条件: Diamonsil-C18(4.6 mm×150 mm)柱, 流动相为甲醇(含0.2%的乙酸)和丙酮, 梯度洗脱, 流速为0.8 mL/min, 进样量10 mL, 检测波长430 nm。在此条件下, 叶绿素a和叶绿素b与浒苔中的其它化合物分离良好, 叶绿素a、b质量浓度为0.50～500 mg/L具有良好的线性关系(R2=0.999 9), 能对浒苔提取液中的叶绿素a、b进行准确定量。

关键词: 浒苔(Enteromorpha prolifera) 叶绿素高效液相色谱法

DOI：

分类号:

基金项目:国家自然科学基金项目 (20905017, 20602009); 海洋公益性行业科研专项项目(200705011, 200805039); 国家海洋局第一海洋研究所基本科研业务专项项目(GY-022008T32, GY-022008G47)

Determination of chlorophyll a and chlorophyll b in Enteromorpha prolifera by ultrasound-assisted extraction with RP-HPLC

CHENG Hong-yan,CHEN Jun-hui,ZHANG Dao-lai,ZHAO Heng-qiang,ZHENG Li,ZANG Jia-ye,WANG Xiao-ru

Abstract:

A convenient and user-friendly reverse phase-high performance liquid chromatography (RP-HPLC) analysis method was established for the determination of chlorophyll a and chlorophyll b in Enteromorpha prolifera. The following HPLC conditions are follows: Diamonsil-C₁₈ (4.6 mm×150 mm) column is used; MeOH-0.2% acetic acid and acetone as the mobile phase with linear gradient elution are used; flow-rate is 0.8 mL/min, detection length of Vis is 430 nm; injection volume is 10 μL. The results indicated that the developed method can be used for the determination of chlorophyll a and b in E. prolifera with a good resolution and linearity between 0.50～500 mg/L(R²=0.999 9).The method is specific, simple, fast, sensitive and feasible for the determination of chlorophyll a and chlorophyll b, which can eliminate the interference of chlorophyll derivatives in E. prolifera.

Key words: Enteromorpha prolifera Chlorophyll HPLC