| 摘要: |
| 根据线粒体D-loop 序列对舟山群岛附近海域的银鲳(Pampus argenteus)群体(n=24)的遗传多样性进行了研究。通过PCR 技术对线粒体D-loop 序列进行扩增, 获得大小约为500 bp 的扩增产物。PCR产物经纯化并进行序列测定后, 得到了357 bp 的核苷酸片段。在24 个个体中, 共检测到14 个变异位点, 其中8 个转换位点, 5 个颠换位点, 1 个转换与颠换同时存在的位点。运用MEGA 软件计算出不同个体间的遗传距离, 并根据其遗传距离构建了UPGMA 和NJ 系统树。DNASP 软件计算出的单倍型多样性(h)、核苷酸多样性(π)及平均核苷酸差异数(k)分别为0.89、0.007 与2.57。此外, 岐点分布及中性检验显示, 东海银鲳群体在历史上可能经历过种群扩张。研究结果表明, 线粒体D-loop 基因可用于银鲳群体内及群体间遗传多样性的分析。 |
| 关键词: 银鲳(Pampus argenteus) 东海 线粒体DNA D-loop 序列 |
| DOI： |
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| 基金项目:中央级公益性科研院所基本科研业务费资助项目(东2008M14, 东2007Z02) |
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| Genetic diversity analysis of silver pomfret (Pampus argenteus) in the East China Sea based on mtDNA D-loop sequence |
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PENG Shi-ming,SHI Zhao-hong,CHEN Chao,HOU Jun-li
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| Abstract: |
| Genetic diversity of silver pomfret (Pampus argenteus) in the East China Sea was investigated based on mtDNA D-loop sequences. The PCR technique was used to amplify the mtDNA D-loop in 24 individuals of silver pomfret collected from the East China Sea, near the coast waters of Zhoushan Islands. The PCR products were purified and sequenced. The results showed that 357bp nucleotide sequences of partial D-loop gene were obtained (the primer and some of the marginal sequences were excluded). Among the 24 individuals, 14 variation sites were observed, of which there were 8 transition sites, 5 transversion sites and 1 transition-transversion sites. The paiwise genetic distances were computed by MEGA software. The UPGMA and NJ phylogenetic trees were obtained through the cluster analysis over the pairwise genetic distance. The haplotype diversity (h), nucleotide diversity (π) and average number of pairwise nucleotide differences (k) were calculated by DNASP software, which were 0.89, 0.007 and 2.57, respectively. Mismatch distribution analysis and Neutrality tests indicated a possible population expansion in silver pomfret population of the East China Sea. In conclusion, mtDNA D-loop may be one of the genetic markers available for scanning genetic diversity of intra-population and inter-populations of silver pomfret. |
| Key words: Pampus argenteus East China Sea mitochondrial DNA D-loop sequence |
