摘要: |
对菲律宾蛤仔(Ruditapes philippinarum)和杂色蛤仔(Ruditapes variegata)的线粒体16S rRNA 基因和COⅠ基因序列进行了分析。结果表明, 16S 序列共有117 个变异位点, COⅠ序列共有160 个变异位点, COⅠ基因编码的219 个氨基酸序列中有29 个不同。根据16S 序列和COⅠ基因编码的氨基酸序列进行聚类分析, 两种蛤仔与硬壳蛤(Mercenaria mercenaria)之间的聚类结果并不一致, 有必要对缀锦蛤亚科更多的相近种进行序列分析。 |
关键词: 菲律宾蛤仔(Ruditapes philippinarum) 杂色蛤仔(Ruditapes variegata) mtDNA 16S rRNA 基因 COⅠ基因 |
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基金项目:国家高技术研究发展计划(863 计划)项目(2002AA603015);山东省农业良种工程课题“优质高产抗逆贝类良种选育” |
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Molecular phylogeny of the genus Ruditapes (Mollusca: Bivalvia) based on mitochondrial 16S and COI sequences |
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Abstract: |
DNA sequence comparisons of two mitochondrial DNA genes were used to infer phylogenetic relationships of the genus Ruditapes. The 16S rRNA gene displayed 117 variable sites and the COⅠ gene displayed 160. There were 29 variable amino acid residues among the 219 of the COⅠgene. Phylogenetic analysis using sequences of 16S rRNA gene and COⅠgene yielded trees with different topology. More sequences among more species of subfamily Tapetinae would contribute to improve existing classification systems. |
Key words: Ruditapes philippinarum Ruditapes variegata mtDNA 16S rRNA gene COⅠgene |