引用本文:	张蕾,胡光荣,范勇,袁程,杨晓红,李福利.高产油突变藻株Desmodesmus sp. D90G-19 的光合作用特征[J].海洋科学,2013,37(11):21-26.

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*高产油突变藻株Desmodesmus* sp. D90G-19 的光合作用特征**
张蕾¹, 胡光荣², 范勇², 袁程², 杨晓红¹, 李福利²
1.西南大学园艺园林学院南方山地园艺学教育部重点实验室;2.中国科学院青岛生物能源与过程研究所

摘要:

Desmodesmus sp.S-1 藻株经重离子诱变后得到的一株突变体D90G-19, 较其野生型的油脂产率提高了20.6%; 同时对野生型和突变株的光合放氧速率, 色素组成和叶绿素荧光动力学进行了分析。与野生型相比, 突变体D90G-19 的光合作用有如下特点: 1. D90G-19 的光饱和点为500 μmol/(m²·s), 无论是在强光还是在弱光下, D90G-19 的光合作用效率都显著高于其野生型; 2. 突变体D90G-19 对高温的适应性比野生型强; 3.在弱碱性条件下,D90G-19 的光合效率的提升较之野生型更为显著。培养D90G-19 的最佳光照强度应在250～400 μmol/(m²· s)之间, 最适温度在25~30℃, 培养液pH 值则以弱碱性为宜(7

关键词: Desmodesmus sp. 光合放氧速率叶绿素荧光光合作用特征

DOI：

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基金项目:中国科学院国际合作项目(31010103907); 中国科学院西部之光项目(Y106140XBL)

The photosynthetic characteristics of a Desmodesmus sp. Mutant D90G-19 with improved lipid productivity

Abstract:

After induced by heavy-ion beam, a mutant of Desmodesmus sp., D90G-19 was obtained, which had a lipid productivity 20.6% higher than the wild type. The photosynthetic characteristics of mutant D90G-19 was characterized by analysis of photosynthetic oxygen evolution rate, chlorophyll concentration and chlorophyll fluorescence. Compared to the wild type, the D90G-19 had a higher photosynthetic efficiency regardless of high or low light intensity, the D90G-19 had a higher temperature tolerance and the D90G-19 had a more significant increase in photosynthetic efficiency under weak base condition. The optimum light intensity, temperature and pH for D90G-19 were 250-400 μmol/(m²·s), 25-30℃ and 72·s), and the Y(NO) suggested that the damage of photosynthesis system II in D90G-19 was less than that in wild type when they were exposed to high intensity light.

Key words: Desmodesmus sp. photosynthetic oxygen evolution rate Chlorophyll fluorescence photosynthetic characteristics