| 引用本文: | 张晓丽,胡玉珍,李明云,苗 亮,陈 炯,史雨红,陆新江.养殖大黄鱼在自然海区降温不同阶段抗氧化水平及血清酶活性的变化[J].海洋科学,2013,37(11):27-34. |
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| 摘要: |
| 为了解越冬期间水温下降对大黄鱼抗氧化水平和血清酶活性的影响, 作者研究了养殖大黄鱼(Pseudosciaena crocea)冬季海区自然降温不同阶段(20、16、12、10、8℃)肝脏和肌肉中抗氧化水平及血清酶活性的变化。结果显示: 自然降温过程中, 肝脏中3 种抗氧化酶变化趋势不同, 其中SOD 活性呈升高趋势, 8℃时SOD活性最强; POD 活性呈先升高后降低的变化趋势, 16℃时活性最高; CAT 活性呈下降趋势; 而肌肉中这3 种抗氧化酶活性均呈下降趋势。肝脏中GSH 和MDA 含量在16℃时最高; 肌肉中的3 种抗氧化酶活性、GSH 和MDA 含量以及总抗氧化能力(T-AOC)均明显低于肝脏中, 反映了肝脏对清除自由基具有重要作用, 在抗氧化调节方面起到主要作用。血清酶ALT、AST、ALP、LDH 及CK-MB 随水温自然下降酶活性均呈现降低趋势, 即20℃时酶活性最强; CK 和LIP 随着水温降低, 呈先上升再下降的趋势; ADA 和GGT 随水温降低活性呈上升趋势。血清酶的不同变化, 说明水温对大黄鱼血清酶活性有一定的影响。 |
| 关键词: 大黄鱼(Pseudosciaena crocea) 自然降温 肝脏 肌肉 抗氧化水平 血清酶活性 |
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| 基金项目:国家高技术研究发展计划(863)项目(2012AA10A403-4); 浙江省重大科技专项(2012C12907-8, 201212C907-9); 宁波市自然科学基金(2013A610162); 长江学者和创新团队发展计划项目(IRT0734) |
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| Changes in antioxidant level and serum enzyme activity of farmed large yellow croaker during natural water cooling in winter |
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| Abstract: |
| In order to investigate the effects of natural water cooling on antioxidant level and serum enzyme activity in farmed large yellow croaker (Pseudosciaena crocea) in winter, the changes of antioxidant level and serum enzyme activity were examined at different stages of natural cooling (20 , 16 , 12 , 10 ℃ ℃ ℃ ℃, and 8℃). The results showed that in liver, the activity of SOD exhibited an increase trend with the highest activity at 8℃; the activity of POD increased first and then decreased, with the highest activity at 16℃; the activity of CAT showed a decrease trend. While in muscle, the activities of these three antioxidant enzymes all decreased during natural water cooling. In liver, the highest concentration of GSH and MDA and the maximum value of total antioxidant capacity (T-AOC) were all observed at 16℃. The activity of SOD, POD and CAT, the concentration of GSH and MDA and the value of T-AOC in liver, were all higher than those in muscle, suggesting the critical roles of liver in free radical scavenging and antioxidation regulation. The activity of serum enzymes (ALT, AST, ALP, LDH and CK-MB) all showed a declining trend, and the highest activities at 20℃. The activity of CK and LIP increased at first and then decreased with decreased water temperature, while ADA and GGT revealed an increasing trend. These data suggest that water temperature impacts on the serum enzyme activities in P. crocea. |
| Key words: large yellow croaker (Pseudosciaena crocea) natural cooling liver muscle antioxidant level serum enzyme activity |
