| 摘要: |
| 采用超声波细胞破碎和索氏回流相结合的方法对马尾藻(Sargassum sp.)总脂质进行了提取, 对提取过程中的超声波细胞破碎功率和时间、料液比、溶剂等关键因素进行了优化; 对总脂质中的多不饱和脂肪酸进行了分离, 并分析了马尾藻脂肪酸组成成分和含量。结果显示, 总脂质的最佳提取条件为超声破碎功率80 W、破碎时间20 min、料液比(m/m)1: 8、石油醚(60～90℃)为溶剂、85℃水浴回流8 h;总脂质最高提取率为1.77%, 其中总脂肪酸平均含量为30.84%; 总脂肪酸中共含有11 种成分, 多不饱和脂肪酸含量约占13.21%～14.60%; 薄层色谱分离出2 种多不饱和脂肪酸C18: 2ω6 和C20: 4ω6。 |
| 关键词: 马尾藻(Sargassum sp.) 多不饱和脂肪酸 超声波细胞破碎 索氏提取 分离 |
| DOI：10.11759/hykx20130422001 |
| 分类号: |
| 基金项目:广西自然科学基金项目(桂科自0991076/2011GXNSFE018002); 广西科学院基本科研业务费项目(09YJ17HY03/11YJ24HY03) |
|
| Extraction, isolation and component analysis of polyunsaturated fatty acids from Sargassum sp. |
|
|
| Abstract: |
| The total lipids of Sargassum sp. were extracted by using ultrasonic cell-break and Soxhlet Extraction. The effects of the power and time of ultrasonic cell-break, ratio of sample and solution, and solvents on total lipid extracting rate were optimized. Furthermore, the polyunsaturated fatty acid was isolated from the total lipid by the procedure of saponifying, esterifying, depigmenting, and thin-layer chromatography separating, and the component of all samples were analyzed by GC-MS. The results showed that the optimal extracting factors of total lipid were ultrasonic power 80W, breaking time of 20 min, ratio of sample to solution of 1: 8, solvent of petroleum ether, and Soxhlet temperature at 85 ℃ for 8 h. The rate of total lipid extracting was up to 1.77%, and the ratio of total fatty acid to TL was 30.84%. There were 11 elements appeared in GC-MS traces in all extracted samples, and the ratio of PUFA to total fatty acid was in the range from 13.21% to 14.60%. Two types of PUFAs, C18: 2ω6 and C20: 4ω6, were obtained through Ag-silicon thin layer chromatography. |
| Key words: Sargassum sp. polyunsaturated fatty acid ultrasonic cell-break Soxhlet Extraction isolation |
