| 摘要: |
| 为探究刺参(Apostichopus japonicus)繁殖期消化、代谢生理特点, 作者以刺参亲参(240 g±15 g)为实验材料, 设置了5 个温度水平(6、10、14、18、21℃), 研究了温度对刺参繁殖期内肠道消化酶和体壁肌肉代谢酶活力的影响。结果发现: 温度对亲参繁殖期消化酶、代谢酶活力受培育水温影响显著(P<0.05)。其中, 胰蛋白酶与淀粉酶活力随水温升高呈先升高后降低的趋势, 在10℃时活力最高且达到峰值; 脂肪酶活力随着养殖水温升高呈下降趋势, 在6℃时活力最高。己糖激酶、丙酮酸激酶、乳酸脱氢酶、苹果酸脱氢酶活力随水温升高出现先增高后降低的趋势, 在10℃时达到峰值; 而琥珀酸脱氢酶活力随温度升高呈下降趋势。研究表明, 10～14℃温度下, 亲参消化酶与代谢酶活力均处于较高水平,是理想的亲参室内促熟温度参数。 |
| 关键词: 刺参(Apostichopus japonicus) 亲参 温度 消化酶 代谢酶 |
| DOI：10.11759/hykx20141020001 |
| 分类号: |
| 基金项目:国家“863”高技术研究发展计划(2012AA10A412); 山东省农业良种工程课题-速生抗病耐高温刺参良种选育 |
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| Effects of temperature on digestive and metabolic enzymes activities of sea cucumber (Apostichopus japonicus) broodstock |
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| Abstract: |
| To investigate the digestive and metabolic performance of adult sea cucumber (Apostichopus japonicus) during breeding season, the activities of digestive and metabolic enzymes including trypsin, lipase, amylase, hexokinase, pyruvate kinase, lactate dehydrogenase, succinate dehydrogenase and malate dehydrogenase of sea cucumber broodstock cultured at different water temperatures (6, 10, 14, 18 and 21℃) were detected. The results indicated that digestive and metabolic enzymes activities were affected by water temperature significantly (P<0.05). The activities of trypsin, amylase, hexokinase, pyruvate kinase, lactate dehydrogenase and malate dehydrogenase increased when the water temperature increased from 6 to 10℃, then decreased when the water temperature increased from 14 to 20℃, and the highest activities were observed at 10℃. While the activities of lipase and succinate dehydrogenase decreased as water temperature rose. Based on results of this study, the optimal temperature range for gonad development of sea cucumber is from 10 to 14℃. |
| Key words: sea cucumber broodstock temperature digestive enzymes metabolic enzymes |
