| 摘要: |
| 为研究青蛤(Cyclina sinensis)对重金属Cu2+的蓄积作用及免疫机能的影响, 开展了Cu2+对青蛤的急性毒性实验, 观察青蛤在96 h Cu2+半致死浓度和安全浓度胁迫下, 不同组织的蓄积趋势, 及血淋巴液中SOD、CAT 和ACP 活性的变化。结果显示: Cu2+的半致死浓度为0.807 mg/L, 安全浓度为0.00807 mg/L。鳃和内脏团组织中蓄积的Cu2+浓度与处理时间呈正相关; 在半致死和安全浓度胁迫下,鳃组织中Cu2+蓄积速度均快于内脏团; 在半致死浓度胁迫下血淋巴液中SOD、CAT 和ACP 活性呈现先诱导再抑制的趋势, 安全浓度下无明显变化, 但均高于对照组水平。 |
| 关键词: 青蛤(Cyclina sinensis) Cu2+ 急性毒性 蓄积过程 免疫指标 |
| DOI：10.11759/hykx20150803004 |
| 分类号: |
| 基金项目:国家贝类产业技术体系项目(CARS-48); 天津市高等学校创新团队基金项目(TD12-5018); 天津市应用基础与前沿技术研究计划(15JCYBJC30400); 天津市科技特派员项目(14JCTPJC00521); 天津市海洋局项目(19-3BC2014-08) |
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| Effects of Cu2+ stress on Cyclina sinensis |
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| Abstract: |
| The acute toxicity test was carried out to determine the effects of Cu2+ on the cumulative action and immune function of Cyclina sinensis. Cu2+ accumulation tendency and hemolymph varation of SOD, CAT and ACP in tissues and immune index of C. sinensis were observed with half lethal concentration (LC50) and safe concentration of Cu2+ for 96 h. The results indicated that the LC50 and safe concentrations of Cu2+ were 0.807 mg/L and 0.00807 mg/L, respectively. The concentrations of Cu2+ were positively correlated with tolerance time in gill and viscus mass. Accumulation rate of Cu2+ in gill is faster than that in the viscus when tested under LC50 and safe concentration of Cu2+. The values of SOD, CAT and ACP in blood presented a variation trend, which were firstly induced, and then inhibited at the LC50 concentration of Cu2+. There were no significant differences between SOD, CAT and ACP at the safe concentration of Cu2+, however, they were all higher than those in control group. This study provides basic information for healthy aquaculture and toxicology research for C. sinensis. |
| Key words: Cyclina sinensis Cu2+ acute toxicity cumulative action immune index |
