引用本文:	迟艳红,陈华增,杨翠华,孙玉苗,齐继光,刘光兴,王玮.桃花水母的核糖体RNA基因ITS区序列分析及物种鉴定[J].海洋科学,2016,40(2):35-40.

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桃花水母的核糖体RNA基因ITS区序列分析及物种鉴定
迟艳红¹, 陈华增¹, 杨翠华¹, 孙玉苗², 齐继光¹, 刘光兴³, 王玮¹
1.青岛海产博物馆;2.中国科学院海洋研究所;3.中国海洋大学环境科学与工程学院

摘要:

利用PCR扩增和DNA测序技术, 获得了青岛野外采集到的桃花水母(Craspedacusta)核糖体RNA基因ITS区序列。序列比对以及系统进化分析显示, 在青岛采集到的桃花水母与索氏桃花水母(Craspedacusta sowerbyi)的ITS区序列相似度最高,同源性在93%以上;同时, 在系统发育树中也与索氏桃花水母聚为一支。研究结果表明, 青岛采集到的桃花水母为索氏桃花水母, 这也是青岛地区桃花水母的新记录。

关键词: 桃花水母(Craspedacusta) 核糖体RNA 基因ITS 区基因克隆序列分析

DOI：10.11759/hykx20150305001

分类号:

基金项目:科技基础性工作专项项目(2012FY112200)

Sequence analysis of the ribosomal RNA gene internal transcribed spacer region and species identification of Craspedacusta

CHI Yan-hong,CHEN Hua-zeng,YANG Cui-hua,SUN Yu-miao,Qi Ji-guang,LIU Guang-xing,WANG Wei

Abstract:

The ribosomal RNA gene internal transcribed spacer (ITS) region of Craspedacusta from Qingdao was isolated using the polymerase chain reaction followed by sequencing. Homology and phylogenetic analyses revealed that the ITS region of Craspedacusta showed a high similarity with Craspedacusta sowerbyi (>93%). Simultaneously, our results showed that this region is closely related to C. sowerbyi using a phylogenetic tree. The present data suggest that the Craspedacusta from Qingdao are closely related to C. sowerbyi. This is a new study about Craspedacusta in Qingdao.

Key words: Craspedacusta ITS region Gene cloning Sequence analysis