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引用本文:刘洪艳,王红玉,谢丽霞,王广策.海洋沉积物中一株铁还原细菌分离及Fe(Ⅲ)还原性质[J].海洋科学,2016,40(3):65-70.
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海洋沉积物中一株铁还原细菌分离及Fe(Ⅲ)还原性质
刘洪艳1, 王红玉1, 谢丽霞1, 王广策2
1.天津市海洋资源与化学重点实验室, 天津科技大学 海洋与环境学院;2.中国科学院海洋研究所
摘要:
以渤海(塘沽海域)沉积物为材料, 富集异化铁还原混合菌群。采用三层平板方法, 从混合菌群中纯化出一株异化铁还原细菌KB52。通过形态观察和16S rRNA基因序列分析, 菌株命名为Klebsiella sp.KB52(GeneBank 号KM233642)。在葡萄糖为电子供体, Fe(OH)3为电子受体条件下厌氧培养菌株KB52,其细胞生长和Fe(Ⅲ)还原具有明显耦合关系。碳源分别设置为葡萄糖、乳酸钠、丙酮酸钠、乙酸钠、甲酸钠和丙酸钠, 在海水培养条件下菌株KB52 以丙酮酸钠为碳源时, 菌株培养液累积Fe(Ⅱ)浓度最高,为4.41 mmol/L±0.59 mmol/L。菌株KB52 在设定NaCl浓度范围内, 都能够生长并具有铁还原性质, 菌株表现出较强的耐盐性。NaCl质量浓度为4 g/L时, 菌株KB52还原Fe(Ⅲ)效率最高, Fe(Ⅱ)达到4.95 mmol/L±0.72 mmol/L。铁还原细菌KB52在淡水和海水条件下能够生长并具有铁还原性质, 可用于近海沉积物中微生物介导异化Fe(Ⅲ)还原过程, 进一步应用于治理海洋环境污染。
关键词:  海洋沉积物  铁还原细菌  Klebsiella sp. KB52  Fe(Ⅲ)还原  培养条件
DOI:10.11759/hykx20150417001
分类号:
基金项目:天津市自然科学基金 (12JCQNJC04200); 大学生创新创业训练计划 (201510057134)
Isolation and characterization of Fe (Ⅲ)-reducing bacterium Klebsiella sp. KB52 from marine sediment
LIU Hong-yan,WANG Hong-yu,XIE Li-xia,WANG Guang-ce
Abstract:
In this study, we collected marine sediment from the Bohai Sea in Tanggu, and enriched them for isolation with Fe (Ⅲ)-reducing microbial strains. The bacterium strain KB52 was isolated for high Fe (Ⅲ)-reduction and, using light microscopic examination and 16S rRNA gene sequence analysis, we identified this strain as Klebsiella sp. KB52 (GeneBank KM233642). Under anaerobic conditions using glucose as an electron donor and Fe (OH)3 as an electron acceptor for Fe (Ⅲ) reduction, Fe (Ⅲ) reduction and cell growth of strain KB52 was synchronous. We investigated the effects of carbon sources, including glucose, lactate, pyruvate, acetate, formate, and propionate, on Fe (Ⅲ)-reducing activity and cell growth in the strain KB52. The results indicated that strain KB52 was able to transfer electrons to Fe (Ⅲ) using various carbon sources and showed a higher Fe (Ⅲ)-reducing activity of 4.41 mmol/L±0.59 mmol/L-Fe (Ⅱ) by adopting pyruvate. In addition, strain KB52 could grow and reduce Fe (Ⅲ) at tested ranges of NaCl concentration from 0 to 60 g/L. It also showed a strong tolerance to salt concentrations. The highest Fe (Ⅲ)-reducing activity of the strain KB52 was 4.95 mmol/L±0.72 mmol/L-Fe (Ⅱ) at an NaCl concentration of 4 g/L. Strain KB52 can grow and tolerate Fe (Ⅲ)-reducing activity under fresh water and marine conditions, which indicates that it can be used as a model organism in the study of Fe (Ⅲ)-reducing activity in isolation from the marine environment. Microbial Fe (Ⅲ) reduction by this strain will contribute to the purification of pollutants and has application to marine environmental protection.
Key words:  marine sediment  Fe (Ⅲ)-reducing bacterium  Klebsiella sp. KB52  Fe (Ⅲ) reduction  culture condition
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