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| 渤海不同区域沉积物古菌的多样性分析 |
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王炳臣1,2, 郑世玲1, 张洪霞1,2, 魏文超1,2, 王欧美3, 刘芳华1
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1.中国科学院 烟台海岸带研究所, 海岸带生物学与生物资源利用所重点实验室;2.中国科学院大学;3.滨州医学院
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| 摘要: |
| 产甲烷古菌在滨海沉积物碳循环过程中起着重要作用。本研究以渤海不同区域沉积物为研究对象, 通过T-RFLP以及室内富集培养方法, 解析了渤海不同区域沉积物产甲烷菌群落结构差异, 并评估其产甲烷潜力。结果表明, 渤海不同区域沉积物细菌和产甲烷古菌群落结构组成存在差异, 其主要优势产甲烷菌为Methanobacterium和, 主要优势细菌为和。渤海不同区域沉积物在实验室培养条件下产甲烷量存在明显差异, 表现为近岸区域沉积物产甲烷量较高。且渤海沉积物具有较高的产甲烷潜力 (乙酸转换率达到46.46 %), 潜在甲烷排放量估计值可达1.74 亿t/年。 |
| 关键词: 甲烷 渤海 产甲烷菌 多样性 产甲烷潜力 |
| DOI：10.11759/hykx20161024002 |
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| 基金项目:国家自然科学基金面上项目(41371257、41573071)、国家自然科学基金青年项目(41401285、31600370), 中国科学院重点部署项目(KZZD-EW-14); 中国科学院百人计划经费资助项目(A 类) |
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| Diversity of archaea in the sediments from different areas of the Bohai Sea |
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WANG Bing-chen,ZHENG Shi-ling,ZHANG Hong-xia,WEI Wen-chao,WANG Ou-mei,LIU Fang-hua
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| Abstract: |
| Methanogens play an important role in the carbon cycle of coastal sediments. In this study, sea water enrichment cultures with acetate as the substrate were employed to enrich methanogens using sediments of the Bohai Sea. The methane production was used to estimate potential methane productivity and terminal restriction fragment length polymorphism analysis was used to analyze the diversity of microbial communities simultaneously. Our results revealed that changes in the bacterial and the methanogens community structures in sediments from different areas of the Bohai Sea were found. The dominant methanogens were Methanobacterium and Methanolobus in sediments of the Bohai Sea, and Desulfovibrio and Thiobacillus were the dominant bacteria. Different areas of the Bohai Sea obviously varied on methane production, which shows that the methane emission is higher in the coastal area. Bohai Sea has great potential for methane productivity and acetic acid conversion rate (46.46%), and the estimation of potential methane production is 1.74 Gt per year. |
| Key words: methane Bohai Sea methanogens diversity potential methane productivity |