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低氧胁迫对菲律宾蛤仔抗氧化酶的影响
聂鸿涛, 卢长炜, 柴成林, 杨 凤, 闫喜武
大连海洋大学, 水产与生命学院, 辽宁省贝类良种繁育工程技术研究中心
摘要:
为了研究不同低氧水平对菲律宾蛤仔(Ruditapes philippinarum)的影响, 在实验室条件下设置溶解氧为0.5 mg/L和2.0 mg/L的低氧海水环境, 以溶氧水平为7.0 mg/L的天然海水为对照组, 研究了低氧胁迫对菲律宾蛤仔抗氧化酶的影响。结果表明: 在低氧胁迫的时间越长的条件下, 乳酸脱氢LDH 和琥珀酸脱氢酶SDH的活力逐渐降低, 而碱性磷酸酶AKP 活力逐渐增大; 对于相同的酶, 对照组的酶活力高于处理组的酶活力; 在溶氧DO越低的情况下, 菲律宾蛤仔的死亡率越高。
关键词:  菲律宾蛤仔(Ruditapes philippinarum)  低氧  胁迫  抗氧化酶
DOI:10.11759/hykx20170715005
分类号:
基金项目:现代农业产业技术体系建设专项(CARS-49); 2017年高等教育内涵建设专项资金(500917201013); 大连市青年科技之星项目(2016RQ065)
Response of the Manila clam antioxidant enzyme to hypoxia stress under experimental conditions
NIE Hong-tao, LU Chang-wei, CHAI Cheng-lin, YANG Feng, YAN Xi-wu
Abstract:
In order to study the effect of different hypoxia levels on the Manila clam Ruditapes philippinarum, we set the dissolved oxygen (DO: 0.5 mg/L) and (DO: 2.0 mg/L) under laboratory hypoxia conditions, and natural seawater (DO: 7.0 mg/L) as the control group. The responses of the R. philippinarum antioxidant enzyme to hypoxia stress under experimental conditions were investigated in the present study. The results showed that the activities of lactate dehydrogenase (LDH) and succinate dehydrogenase (SDH) decreased gradually in the longer hypoxia stress conditions, but the activity of alkaline phosphatase (AKP) increased gradually. The activity of the same enzyme in the control group was higher than in the hypoxia treatment group. There were lower levels of dissolved oxygen and higher mortality rates in R. philippinarum. The results provide a biological foundation for the physiological ecology of the R. philippinarum under low oxygen environment.
Key words:  Ruditapes philippinarum  Hypoxia  Stress  antioxidant enzyme
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