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海洋酸化对青蛤免疫指标的影响
梁 健1, 高 山1, 李永仁1, 殷 潇1, 刘金桥1, 郭永军1, 闫喜武2
1.天津农学院 水产学院, 天津市水产生态及养殖重点实验室;2.大连海洋大学, 辽宁省贝类良种繁育工程技术研究中心
摘要:
通过向水体中通入CO2的方法模拟海洋酸化环境, 测定青蛤(Cyclina sinensis)在酸化条件下各免疫指标的变化情况。结果显示: 将青蛤置于酸化的海中(pH分别为7.4和7.7), 并以自然海水做对照组(pH 8.1)后, 血细胞总数随海水酸化胁迫时间的延长, 表现为下降趋势, 且差异显著(P<0.05); 海水pH降低抑制了溶菌酶的活性, 但差异性不显著(P>0.05); ACP活性总体呈下降趋势, 对照组活性要高于酸化组, 而ALP活性表现为上升趋势。酸化胁迫初期诱导SOD活性升高, 后期SOD活性受到抑制, 而CAT变化却截然相反; 脂质过氧化产物MDA在酸化后期出现显著降低(P<0.05)。
关键词:  海洋酸化  青蛤(Cyclina sinensis)  免疫
DOI:10.11759/hykx20170715002
分类号:
基金项目:天津市现代产业技术体系-水产-贝类养殖岗位(ITTFRS2017013);现代农业产业技术体系建设专项资金(CARS-49); 天津市农业科技成果转化与推广项目(201502350); 天津市科技特派员项目(16JCTPJC46200);天津市应用基础与前沿技术研究计划(15JCYBJC30400); 天津市科技计划项目(15ZXZYNC00010)
Effect of ocean acidification on the immune index of Cyclina sinensis
LIANG Jian,GAO Shan,LI Yong-ren,YIN Xiao,LIU Jin-qiao,GUO Yong-jun,YAN Xi-wu
Abstract:
To measure the immune index of Cyclina sinensis under acidification environments, CO2 was dissolved into the seawater. It was found that the total blood cells count had a significantly decreasing trend with increasing acidification time (P < 0.05); decrease in pH levels inhibited the activity of lysozyme, but the difference was not statistically significant (P > 0.05). The ACP activity was generally deceased, but higher in the control group than the acidification group. However, the ALP activity showed an upward trend. On the other hand, the activity of SOD increased during the initial stages of acidification stress but was inhibited in the later stages. However, the opposite trend was observed for CAT activity; lipid peroxidation products-MDA decreased significantly in the later stage of acidification (P < 0.05).
Key words:  Ocean acidification  Cyclina sinensis  Immune index
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