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肠道细菌对厚壳贻贝稚贝附着的作用研究
杨 娜1,2, 梁 箫1,2,3, 彭莉华1,2, 沈和定1,2,3, 杨金龙1,2,3
1.上海海洋大学 海洋生物科学国际联合研究中心;2.上海海洋大学 水产种质资源发掘与利用教育部重点实验室;3.上海海洋大学 水产科学国家级实验教学示范中心
摘要:
为探讨肠道细菌形成的微生物被膜对贝类附着的作用, 从厚壳贻贝(Mytilus coruscus)成体肠道中分离10株肠道细菌, 调查厚壳贻贝肠道细菌形成微生物被膜特性, 分析微生物被膜密度、细菌种属与厚壳贻贝稚贝附着之间的相互关系。所有肠道细菌均可有效促进稚贝附着。其中, Paracoccus sp. 1微生物被膜诱导的稚贝附着率最高(61%), 且微生物被膜膜厚较厚, 细菌分布较密集; 仅5株肠道细菌Roseovarius sp. 1、Winogradskyella sp. 1、Tenacibaculum sp. 4、Bacillus sp. 5和Nonlabens sp. 1的被膜密度与附着显著相关(P< 0.05)。系统发育分析发现肠道细菌诱导附着率与其种属关。本研究表明源于厚壳贻贝成贝肠道细菌可以促进其稚贝的附着, 说明肠道细菌对厚壳贻贝的生长发育具有一定的作用。本研究结果为深入探讨肠道细菌与厚壳贻贝相互作用和推动该种规模化养殖提供理论依据。
关键词:  厚壳贻贝  稚贝  附着  肠道细菌  微生物被膜
DOI:10.11759/hykx20170626001
分类号:
基金项目:国家自然科学基金(41476131); 上海高校水产高峰学科建设项目(水产科学)
Effects of gut bacteria on the settlement of spats of Mytilus coruscus
YANG Na,LIANG Xiao,PENG Li-hua,SHEN He-ding,YANG Jin-long
Abstract:
Mytilus coruscus is an important marine commercial mollusc in southeast coastal areas of China due to its high nutritional and dietary value, while overfishing has resulted in the increasing scarcity of wild mussel resources. Thus, improving the technique of artificial culture in this species is necessary. Various biofilms have been well-recognized to play essential roles in the settlement of larvae and juveniles of many species of invertebrates. However, the relationship between gut bacterial biofilm and settlement of invertebrates is poorly characterized. In this study, to understand settlement of the mussel M. coruscus in response to the different bacterial strains isolated from the gut of M. coruscus adults, the interaction between bacterial biofilms and settlement was investigated. In addition, the phylogenetic tree derived from the selected 16S rDNA sequence was constructed and the genetic distance was calculated. The results showed that the density of monospecific bacterial biofilms increased with the initial incubation density and all test bacterial biofilms showed significantly inducing activity on settlement of M. coruscus spats. Among the 10 bacterial species, the biofilm of Paracoccus sp. 1 induced the highest percentage of settlement of 61%, and biofilm thickness relatively was thick and intensive. There was signification correlation between the settlement and the cell densities of biofilms of Roseovarius sp. 1, Winogradskyella sp. 1, Tenacibaculum sp. 4, Nonlabens sp. 1 and Bacillus sp. 5. The inducing activity of individual bacterial isolates was not correlated with their phylogenetic relationship. Thus, gut bacteria can promote the settlement of M. coruscus, and may have a positive effect on the growth and development of M. coruscus. The findings will provide a novel insight into understanding the relationship between gut bacteria and the growth and development of M. coruscus, and also are helpful for enhancing the artificial culture of the species.
Key words:  Mytilus coruscus  spat  settlement  gut bacteria  biofilm
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