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氯化锌对松江鲈肾细胞系的毒性效应
梁 艳1, 白丽雯1, 李 霞1,2, 秦艳杰1, 吴 迪1, 周诗珈1
1.大连海洋大学 辽宁省海洋生物资源恢复与生境修复重点实验室;2.大连海洋大学农业部北方海水增养殖重点实验室
摘要:
本文研究了不同浓度的氯化锌对55~65代松江鲈(Trachidermus fasciatus)肾细胞系的毒性效应。采用MTT法测得氯化锌24 h半致死浓度为308.41 μmol/L。酶活力测定的结果显示: 超氧化物歧化酶(SOD)和谷胱甘肽-S-转移酶(GST)的活性在氯化锌浓度为0~250 μmol/L时, 随浓度的升高逐渐升高;在250 μmol/L浓度时达到最大值, 而后随着氯化锌浓度的升高逐渐降低。谷胱甘肽过氧化物酶(GSH-Px)的活性在氯化锌浓度为125 μmol/L浓度时达到最大值, 而后随着氯化锌浓度的升高逐渐降低。微核实验结果是微核率随氯化锌浓度增加而增加, 最高达12.33‰。彗星实验发现在半致死浓度条件下松江鲈肾细胞拖尾率为48.00%、迁移长度15.03 μm±2.51 μm, 与对照组差异显著(P<0.05)。以上结果表明: 氯化锌会引起肾细胞氧化酶活性的改变以及细胞核DNA 损伤, 本研究认为松江鲈肾细胞系可以作为Zn2+污染的监测平台。
关键词:  松江鲈(Trachidermus fasciatus)  肾细胞系  氯化锌  半致死浓度  酶活力  DNA 损伤
DOI:10.11759/hykx20160617001
分类号:
基金项目:国家自然科学基金项目(31272650)
Study on the cytotoxicity of zinc chloride to Trachidermus fasciatus kidney cell lines in vitro
LIANG Yan,BAI Li-wen,LI Xia,QIN Yan-jie,WU Di,Zhou Shi-jia
Abstract:
This study investigated the toxicity of zinc chloride in vitro administered to the kidney cell line of Trachidermus fasciatus. The 24-h LC50 of zinc chloride was 308.41 μmol/L as estimated by the MTT assay. The SOD activity and the GST activity of the kidney cells gradually increased with increasing concentrations of zinc chloride over the range of 0–250 μmol/L, reaching a maximum at 250 μmol/L, and then gradually decreased. The GSH-Px activity reached a maximum at 125 μmol/L and then gradually decreased. The micronucleus test indicated that kidney cells produced the micronucleus, and the micronucleus rates gradually increased with increasing concentrations of zinc chloride; the highest micronucleus rate was 12.33‰. The comet assay showed that zinc chloride could cause nucleus damage. The comet rate was 48.00%, and the comet length of cellular DNA was 15.03 μm ± 2.51 μm. Both the micronucleus test and the comet assay results showed that there were genotoxic effects on the kidney cell line of T. fasciatus when exposed to zinc chloride. The kidney cell line of T. fasciatus could be used to monitor the contamination by zinc as a bioindicator for in coasta.
Key words:  Trachidermus fasciatus  kidney cell line  zinc chloride  semi-lethal concentration  enzyme activity  DNA damage
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