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中国近海大型底栖动物DNA条形码的研究进展 |
杨梅1,2,3, 寇琦1,2,3,4, 李新正1,2,3,4
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1.中国科学院海洋研究所海洋生物分类与系统演化实验室, 山东 青岛 266071;2.中国科学院海洋大科学研究中心, 山东 青岛 266071;3.青岛海洋科学与技术国家实验室 海洋生物学与生物技术功能实验室, 山东 青岛 266237;4.中国科学院大学, 北京 100049
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摘要: |
DNA条形码是利用标准的、变异率适度、易于扩增且相对较短的DNA片段对物种进行快速准确鉴定的技术。海洋大型底栖动物分布广泛,具有多样性、复杂性和趋同性等特点,DNA条形码技术能够在传统分类学基础上对物种进行快速、准确地鉴定。本文概述了DNA条形码技术在中国近海大型底栖动物研究中的发展现状,如多孔动物(Porifera)、刺胞动物(Coelenterata)、多毛动物(Polychaeta)、软体动物(Mollusca)、甲壳动物(Crustacea)、棘皮动物(Echinodermata)等,介绍了该技术在物种鉴定、隐存种发现、生物多样性评估等领域的研究进展,指出了目前条形码技术在应用中的局限性,并对未来的研究工作进行了展望。 |
关键词: 大型底栖动物 DNA条形码 分类学 物种鉴定 |
DOI:10.11759/hykx20180726002 |
分类号:Q959 |
基金项目:国家自然科学基金(41506173);科技部基础性工作专项(2014FY110500) |
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Advances in DNA barcoding of macrozoobenthos in coastal waters of China |
YANG Mei1,2,3, KOU Qi1,2,3,4, LI Xin-zheng1,2,3,4
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1.Department of Marine Organism Taxonomy and Phylogeny, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China;2.Center for Ocean Mega-Science, Chinese Academy of Sciences, Qingdao 266071, China;3.Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266237, China;4.University of Chinese Academy of Sciences, Beijing 100049, China
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Abstract: |
DNA barcoding is a technology utilized for accurate and rapid identification of species by analyzing standard short DNA sequences with sufficient variation in organism. Macrobenthos communities are widely distributed in the ocean, with high diversity, complexity and homoplasy. Combined with traditional morphological methodology, DNA barcoding can identify species rapidly and accurately. In this paper, we summarize the current developments in DNA barcoding of Macrozoobenthos (i.e., Porifera, Cnidaria, Polychaeta, Mollusca, Crustacea, and Echinodermata) in coastal waters of China and introduce its advances in species identification, cryptic species, and biodiversity assessment. We also discuss the limitations and future prospect of DNA barcoding. |
Key words: Macrozoobenthos DNA barcoding taxonomy species identification |