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引用本文:夏苏杭,全艳玲,周艳文,陈东旭,康宏伟,吕哲,陈爽,高乙宁,李娜,解生权,高鹏.海洋污损细菌分离纯化及金属离子抑菌研究[J].海洋科学,2020,44(1):75-80.
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海洋污损细菌分离纯化及金属离子抑菌研究
夏苏杭1, 全艳玲1, 周艳文1, 陈东旭1, 康宏伟1, 吕哲1, 陈爽1, 高乙宁1, 李娜1, 解生权2, 高鹏3
1.辽宁科技大学 化学工程学院 表面工程研究所, 辽宁 鞍山 114051;2.辽宁经济职业技术学院, 辽宁 沈阳 110122;3.海洋装备用金属材料及其应用国家重点实验室, 辽宁 鞍山 114000
摘要:
为了研究海洋生物污损对船体和海洋工程的影响,作者将热喷涂镀钴基、镍基及含铜氧化钛基膜的船用厚钢板试样片放置在海水中,利用振荡培养箱模拟海洋环境,进行晃片培养。分离纯化镀膜钢片表面上的细菌,研究早期污损细菌的生长规律,通过革兰氏染色、生理生化实验对细菌进行鉴定,伯杰氏系统细菌学手册查证。采用纸片法测定抑菌圈,研究铜、钴、镍3种金属离子对污损细菌的影响,观察不同离子浓度的抑菌圈。研究结果表明:早期海洋污损细菌有G+芽孢杆菌属(Bacillus)、G+芽孢乳杆菌属(Sporolactobacillus)、G+无芽孢杆菌(Bacilli)、葡萄球菌属(Staphylococcus)、G+无芽孢丝状菌、假单胞菌属(Pseudomonas)6类;其中G+细菌首先吸附;Cu2+的最佳抑制质量浓度为30 g/L,Co2+的最佳抑制质量浓度为80 g/L,Ni2+的最佳抑制质量浓度为110 g/L。
关键词:  海洋污损  早期污损细菌  金属离子  抑菌作用
DOI:10.11759/hykx20190620001
分类号:Q938.8
基金项目:辽宁省科技厅资助项目(No.SKLMEA-USTL-201709)
Separation and purification of marine fouling bacteria and inhibition of metal ions
XIA Su-hang1, QUAN Yan-ling1, ZHOU Yan-wen1, CHEN Dong-xu1, KANG Hong-wei1, LV Zhe1, CHEN Shuang1, GAO Yi-ning1, LI Na1, XIE Sheng-quan2, GAO Peng3
1.College of Chemical Engineering, Surface Engineering Institute, University of Science and Technology Liaoning, Anshan 114051, China;2.LiaoningVocational Institute of Economics, Shenyang 110122, China;3.State Key Laboratory of Marine Equipment Steel and its Application, Anshan Iron and Steel Research Institute, Anshan 114000, China
Abstract:
Marine biofouling has dramatic impacts on hull and ocean engineering. Samples of thick marine steel plates hot sprayed with cobalt-, nickel-, and copper-titanium-containing film coatings were placed in sea water. The sea environment was simulated by a shaking incubator. The bacteria on the surface of the coated steel sheet were separated and purified, and the growth curve of early fouling bacteria was studied. The bacteria were identified by Gram staining and physiological and biochemical experiments. Bacteria were also identified according to Berger's systematic bacteriology manual. The inhibition zone was determined by the paper method; the effects of three metal ions, copper, cobalt, and nickel, on one strain of bacteria were studied, and the inhibition zone at different ion concentrations was observed. The results showed that in the early marine fouling stage, the bacteria included G+ Bacillus, G+ non-Bacillus, Staphylococcus, G+ nonspore-forming filamentous fungi, and Pseudomonas; G+ bacteria were adsorbed first. The optimal inhibitory concentrations of Cu2+, Co2+, and Ni2+ were 30, 80, and 110 g/L, respectively.
Key words:  Marine fouling  early fouling bacteria  metal ions  bacteriostasis
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