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引用本文:张思研,王绍军,杨佳程,朱建业,田莹,王荦,毛俊霞,王许波,常亚青,鹿志创,郝振林.盐度对香螺行为、鳃和肾抗氧化酶活性与组织结构的影响[J].海洋科学,2022,46(10):129-139.
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盐度对香螺行为、鳃和肾抗氧化酶活性与组织结构的影响
张思研1, 王绍军2, 杨佳程1, 朱建业1, 田莹1, 王荦1, 毛俊霞1, 王许波1, 常亚青1, 鹿志创3, 郝振林1
1.大连海洋大学农业农村部北方海水增养殖重点实验室, 辽宁 大连 116000;2.山东省渔业发展和资源养护总站, 山东 济南 250000;3.辽宁省海洋水产科学研究院, 辽宁 大连 116000
摘要:
为明确不同盐度胁迫下香螺的生理生态学响应及其适应机制,本文采用行为学、组织学和生物化学手段研究了不同盐度条件下(16、20、24、28、32、36、40、44、48)香螺的行为、病理损伤以及抗氧化酶活性。结果显示,在高盐和低盐胁迫下,香螺出现应激反应,摄食率下降,死亡率升高。随盐度升高,香螺的总抗氧化能力先升高后降低,在盐度32时达到峰值;香螺鳃的过氧化氢酶(CAT)活力和超氧化物歧化酶(SOD)活力呈先降低后升高的趋势;肾的CAT和SOD活力呈先升高后降低的趋势。组织切片结果显示,在高盐和低盐胁迫下,香螺嗅检器和鳃丝的形态呈现显著变化,黏液细胞数量均增加。上述结果表明,盐度显著影响香螺的生理生态反应,且香螺可通过行为变化、调节抗氧化酶以及改变组织形态结构从而适应外界盐度的变化。
关键词:  香螺  盐度  生理胁迫  行为  抗氧化酶活性  病理损伤
DOI:10.11759/hykx20211020004
分类号:
基金项目:国家自然科学基金(42076101);辽宁省自然资源厅海洋经济发展专项;辽宁省教育厅面上项目(LJKZZZ0700);大连市重点研发计划项目(2021YF16SN015)
Effect of salinity on Neptunea cumingii behavior and antioxidant enzyme activities and tissue structure of the gill and kidney
ZHANG Si-yan1, WANG Shao-jun2, YANG Jia-cheng1, ZHU Jian-ye1, TIAN Ying1, WANG Luo1, MAO Jun-xia1, WANG Xu-bo1, CHANG Ya-qing1, LU Zhi-chuang3, HAO Zhen-lin1
1.Key Laboratory of Mariculture & Stock Enhancement in North China's Sea, Ministry of Agriculture and Rural Affairs, Dalian Ocean University, Dalian 116000, China;2.Shandong Fisheries Development and Resource Conservation Center, Jinan 250000, China;3.Liaoning Ocean and Fisheries Science Research Institute, Dalian 116000, China
Abstract:
The behavior, pathological damage, and antioxidant enzyme activities of Neptunea cumingii under different salinity conditions (16, 20, 24, 28, 32, 36, 40, 44, and 48) were studied using different methods to clarify the physiological and ecological response and adaptive mechanism of N. cumingii under different salinities. The results showed that N. cumingii exhibited a stress response, decreased feeding rate, and increased mortality under high and low salt stress. The total antioxidant capacity of N. cumingii first increased and then decreased as salinity increased from 16 to 32. The activities of catalase (CAT) and superoxide dismutase (SOD) in gill first decreased and then increased. Kidney CAT and SOD activities first increased and then decreased. The tissue section results showed that the morphology of the N. cumingii olfactory apparatus and gill filaments changed significantly under high salt and low salt stress, and the number of mucus cells increased. These results show that salinity significantly affected the physiological and ecological responses of N. cumingii. N. cumingii adapted to the external salinity changes by changing behavior, regulating antioxidant enzyme activities, and changing tissue morphological structure.
Key words:  Neptunea cumingii  salinity  behavior  antioxidant enzyme activity  pathological damage
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