引用本文:	刘英,王全超,李海汇,纪莹璐,陈琳琳,李宝泉.小清河河口邻近海域口虾蛄(Oratosquilla oratoria)COI序列特征及遗传多样性分析[J].海洋科学,2022,46(11):38-46.

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*小清河河口邻近海域口虾蛄(Oratosquilla oratoria)COI序列特征及遗传多样性分析*
刘英^1,2, 王全超¹, 李海汇³, 纪莹璐⁴, 陈琳琳¹, 李宝泉^1,2
1.中国科学院烟台海岸带研究所, 山东烟台 264003;2.中国科学院大学, 北京 100049;3.寿光市渔业发展中心, 山东寿光 262700;4.国家海洋局北海预报中心, 山东青岛 266061

摘要:

受河流沿岸工农业发展以及人口急剧增加影响,小清河河口及邻近海域受到严重污染。口虾蛄(Oratosquilla oratoria)作为小清河河口及邻近海域重要渔业资源之一,承受着巨大的捕捞和生存压力。为了掌握口虾蛄种群在该区域的种群遗传多样性,本研究于2020年6—10月在小清河河口及邻近海域采集口虾蛄生物样本,进行了基于线粒体COI基因的种群遗传多样性分析。研究结果表明,基于216条长度为655bp的COI基因片段,共定义90个单倍型,63个多态位点,其中包括59个转换位点,1个颠换位点和3个转换与颠换同时存在的位点,核苷酸多样性指数和单倍型多样性分别为0.9700和0.0051。与其他海域相比,小清河河口种群遗传多样性处于相对较高水平;同时遗传结构分析发现黄渤海与东海及南海分布的口虾蛄种群遗传变异较大,但黄渤海海域内遗传变异较小。通过本研究基本掌握了小清河河口及邻近海域口虾蛄种群遗传多样性背景,也可为口虾蛄资源管理以及种质资源库建立等提供科学的基础理论依据。

关键词: 小清河河口及邻近海域口虾蛄线粒体COI 遗传多样性

DOI：10.11759/hykx20210618001

分类号:S917.4

基金项目:美丽中国生态文明建设科技工程专项(XDA23050304);山东省自然基金面上项目(ZR2021MD084)

Analysis of the COI sequence characteristics and genetic diversity of Oratosquilla oratoria in the Xiaoqing River Estuary and its adjacent sea

LIU Ying^1,2, WANG Quan-chao¹, LI Hai-hui³, JI Ying-lu⁴, CHEN Lin-lin¹, LI Bao-quan^1,2

1.Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Laboratory of Protection of Biological Resources Biology in Coastal Waters, Yantai 264003, China;2.University of Chinese Academy of Sciences, Beijing 100049, China;3.Shouguang Marine Fishery Development Center, Shouguang 262700, China;4.North China Sea Marine Forecasting Center of State Oceanic Administration, Qingdao 266061, China

Abstract:

The Xiaoqing River Estuary has been severely polluted due to the quick development of industry and agriculture as well as the rapid increase in the population in the region. Mantis shrimp (Oratosquilla oratoria) is an important fishery resource in this area that is under great threat from overfishing and pollution. In this study, samples were collected in the Xiaoqing River Estuary and its adjacent sea during June-August 2020 to understand the population genetic diversity based on COI gene. A total of 90 haplotypes and 63 polymorphic loci, including 59 transition loci, 1 transversion loci, and 3 loci where both a transition and transversion existed, were defined based on 216 COI gene fragments with a length of 655 bp. The nucleotide diversity and haplotype diversity were 0.970 0 and 0.005 1, respectively. Compared with other sea areas, we found that the genetic diversity of the Xiaoqing River Estuary population was relatively high. Meanwhile, genetic structure analysis showed an obvious difference in the genetic variation of the mantis shrimp population on a large spatial scale, e.g., the Yellow Sea and the Bohai Sea, the East China Sea and the South China Sea. Whereas, it did not show any difference on a relatively smaller spatial scale, e.g., the Yellow Sea and the Bohai Sea. The genetic diversity background of the mantis shrimp population in the Xiaoqing River Estuary and its adjacent sea could provide a theoretical basis for resource management and germplasm bank establishment.

Key words: Xiaoqing River Estuary and its adjacent sea areas Oratosquilla oratoria COI gene genetic diversity