| 引用本文: | 羌玺,王旭雷,牛建峰,闫舒恒,张玉红,宋玉龄,王广策,宫相忠,王立军.江蓠属不同种质资源中藻胆蛋白和琼胶含量差异及综合提取工艺研究[J].海洋科学,2022,46(11):107-115. |
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| 江蓠属不同种质资源中藻胆蛋白和琼胶含量差异及综合提取工艺研究 |
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羌玺1,2,3, 王旭雷2,4, 牛建峰2,4, 闫舒恒2,4, 张玉红5, 宋玉龄2, 王广策2,4, 宫相忠1, 王立军2,4,3
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1.中国海洋大学海洋生命学院, 山东 青岛 266003;2.中国科学院海洋研究所中国科学院实验海洋生物学重点实验室海洋大科学研究中心, 山东 青岛 266071;3.南通中科海洋科学与技术研究发展中心, 江苏 南通 226334;4.青岛海洋科学与技术试点国家实验室海洋生物学和生物技术功能实验室, 山东 青岛 266237;5.青岛市市南区疾病预防控制中心, 山东 青岛 266072
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| 摘要: |
| 藻胆蛋白和琼胶是来源于藻类的重要活性物质,随着藻胆蛋白和琼胶需求量的日益提升,寻找合适的提取原料尤为重要。本研究比较了中国8种常见江蓠(Gracilaria)的藻胆蛋白和琼胶含量,建立了一种环保的综合提取工艺并进一步优化了琼胶提取条件。研究结果显示不同品种江蓠的藻胆蛋白和琼胶含量有显著差异,张氏江蓠(Gracilaria changii)的藻胆蛋白和琼胶含量相对较高。以张氏江蓠为综合提取藻源,藻蓝蛋白的得率为0.073mg/g,藻红蛋白得率为0.088mg/g,通过单因素实验和正交模型优化琼胶提取工艺,最佳提取工艺为碱浓度4%,碱处理时间2h,碱处理温度65℃,琼胶得率为43.51%。结果表明,张氏江蓠有较高生物资源利用率,综合提取藻胆蛋白和琼胶可以提高张氏江蓠的应用价值,研究为张氏江蓠的综合开发提供理论依据。 |
| 关键词: 江蓠(Gracilaria) 琼胶 藻胆蛋白 综合提取 高值化利用 |
| DOI：10.11759/hykx20220907001 |
| 分类号:P745 |
| 基金项目:国家重点研发计划项目(2018YFD0901500);山东省重大科技创新工程项目(2019JZZY010815);国家现代农业产业技术体系(CARS-50);南通市科技计划项目(MS22021017);山东省“泰山学者”工程专项经费资助项目(tspd20210316) |
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| Optimization of content and comprehensive extraction of phycobiliprotein and carrageenan from Gracilaria |
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QIANG Xi1,2,3, WANG Xu-lei2,4, NIU Jian-feng2,4, YAN Shu-heng2,4, ZHANG Yu-hong5, SONG Yu-ling2, WANG Guang-ce2,4, GONG Xiang-zhong1, WANG Li-jun2,4,3
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1.College of Marine Life Sciences, Ocean University of China, Qingdao 266003, China;2.CAS and Shandong Province Key Laboratory of Experimental Marine Biology, Center for Ocean Mega-Science, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China;3.Nantong Zhong Ke Marine Science and Technology R&D Center, Nantong 226334, China;4.Laboratory for Marine Biology and Biotechnology, Pilot National Laboratory for Marine Science and Technology(Qingdao), Qingdao 266237, China;5.Qingdao Shinan District Center for Disease Control & Prevention, Qingdao 266072, China
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| Abstract: |
| Phycobiliproteins and agar are active compounds isolated from algae, which have widespread applications in various fields. It is vital to select suitable Gracilaria as the raw material for agar extraction, considering the increasing demand and consumption of phycobiliproteins and agar. In this study, phycobiliproteins and agar were extracted from Gracilaria in China for a comparative analysis of the differences in their contents. Additionally, an environmentally friendly comprehensive method for extraction of phycobiliprotein and agar was established. The results demonstrated the differences in contents of phycobiliproteins and agar from eight species of Gracilaria. Gracilaria changii had higher contents of phycobiliproteins and agar. The yields of phycocyanin and phycoerythrin from Gracilaria changii were 0.073 mg/g and 0.088 mg/g, respectively. The extraction conditions of agar were optimized by single factor experiments and an orthogonal model. The yield of agar was 19.60% at 4% alkali concentration, 2 h alkali treatment time, and extraction at 62 ℃. Therefore, this study showed that Gracilaria changii had a higher utilization rate than other seven species. It also showed that the comprehensive extraction method can make better use of Gracilaria changii, which improved its application value. Thus this study provides valuable information required for the development of Gracilaria changii. |
| Key words: Gracilaria agar phycobiliprotein comprehensive extraction high-value utilization |
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