| 引用本文: | 任庆敏,林燕妮,王寅初,秦松.不同铁浓度对一种颗石藻Emiliania huxleyi的生长及光合作用的影响[J].海洋科学,2022,46(12):50-62. |
| |
|
| |
|
|
| 本文已被：浏览 615次 下载 1121次 |
码上扫一扫！ |
|
|
| 不同铁浓度对一种颗石藻Emiliania huxleyi的生长及光合作用的影响 |
|
任庆敏1,2, 林燕妮3, 王寅初1,4,5, 秦松1,4,5
|
|
1.中国科学院烟台海岸带研究所, 山东 烟台 264003;2.中国科学院大学, 北京 100049;3.烟台大学, 山东 烟台 264005;4.国家基础学科公共科学数据中心, 北京 100190;5.中国科学院海洋大科学研究中心, 山东 青岛 266071
|
|
| 摘要: |
| 为探讨铁对颗石藻生长及光合作用的影响, 以分离于秘鲁上升流区域的两株不同基因型的赫胥黎艾氏藻Emiliania huxleyi RCC6660与RCC6666为研究对象, 在调整5种铁浓度(0、50、100、500、1 000 nmol/L)的Aquil培养基中培养, 比较其生长率、色素含量、营养盐消耗、颗粒有机物含量及叶绿素荧光特性参数的差异。实验结果表明: 铁浓度未对两株藻的生长产生显著影响, 但0 nmol/L时RCC6660的比生长率显著高于RCC6666(P < 0.05)。随着铁浓度的减少, 细胞粒径和体积减小, 最大电子传递速率(rETRmax)和表观光能利用效率降低。我们推测RCC6660为了保护细胞在低铁时免受光损伤, 通过叶黄素循环以及高度钙化的外壳实现光保护, 而RCC6666通过更多其他形式的非光化学荧光淬灭的手段。由于钙化是个耗能过程, 低钙化的RCC6666的光能利用效率在低铁浓度下也更高。但RCC6660的rETRmax更大, 拥有较高的光合潜力, 这可能与其拥有的更高含量的色素有关。本研究揭示了不同基因型、不同钙化程度的两株Emiliania huxleyi对不同铁浓度的差异响应, 及铁限制条件下光保护机制和光能利用的不同, 为进一步研究光合和钙化作用的偶联机制提供了参考。 |
| 关键词: 颗石藻 铁限制 光合 叶绿素荧光 |
| DOI：10.11759/hykx20201109002 |
| 分类号:P735;P76 |
| 基金项目:国家基础学科公共科学数据中心“中国海岸带植物资源数据库”(NBSDC-DB-22); 内蒙古自治区关键技术攻关计划(2021GG0414) |
|
| Effects of different iron concentrations on the growth and photosynthesis of Emiliania huxleyi |
|
REN Qing-min1,2, LIN Yan-ni3, WANG Yin-chu1,4,5, QIN Song1,4,5
|
|
1.Key Laboratory of Coastal Biology and Biological Resource Utilization, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai 264003, China;2.University of Chinese Academy of Sciences, Beijing 100049, China;3.Yantai University, Yantai 264005, China;4.National Basic Science Data Center, Beijing 100190, China;5.Center for Ocean Mega-Science, Chinese Academy of Sciences, Qingdao 266071, China
|
| Abstract: |
| Two genotypes of Emiliania huxleyi, RCC6660 and RCC6666, isolated from a Peruvian upwelling, were cultured in Aquil medium at five different iron concentrations (0, 50, 100, 500, and 1, 000 nmol/L) to study the effect of iron on the growth and photosynthesis of coccolithophores. The difference in growth, pigment content, nutrient consumption, particulate organic matter content, and chlorophyll fluorescence characteristics were compared. The results revealed that iron concentration had no significant effect on the growth of the two strains; however, the specific growth rate of RCC6660 in 0 nmol/L was significantly greater than that of RCC6666. As the iron concentration decreased, cell size, cell volume, the maximum relative electron transport rate (rETRmax), and apparent quantum efficiency (α) also decreased. We speculate that RCC6660 was photoprotected by the lutein cycle and a highly calcified exoskeleton that protected the cells from light damage at low iron levels; moreover, RCC6666 was more likely to be quenched by other forms of non-photochemical fluorescence. The light energy efficiency of RCC6666 was higher at low iron concentrations, as less energy was devoted to calcification. However, RCC6660 had a higher rETRmax and photosynthetic potential, which may be related to its higher pigment content. This study revealed the responses of two Emiliania huxleyi strains with different genotypes and different calcification abilities to different iron concentrations and the differences in the photosynthetic protective mechanism and light energy utilization under an iron limitation, which provides a reference for further studies of the coupling mechanism between photosynthesis and calcification. |
| Key words: coccolithophores iron limitation photosynthesis chlorophyll fluorescence |
|
|
|
|
|
|