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引用本文:周慧欣,王锦秀,王馨,徐子钧,颜天.日本虎斑猛水蚤在抑食金球藻中存活、发育和繁殖的实验研究[J].海洋科学,2024,48(3):42-49.
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日本虎斑猛水蚤在抑食金球藻中存活、发育和繁殖的实验研究
周慧欣1,2, 王锦秀1, 王馨1,2, 徐子钧3, 颜天1
1.中国科学院海洋生态与环境科学重点实验室 中国科学院海洋研究所, 山东 青岛 266404;2.中国科学院大学, 北京 100049;3.自然资源部北海生态中心, 山东 青岛 266033
摘要:
抑食金球藻(Aureococus anophagefferens)可以形成褐潮, 并对贝类、浮游动物等多种生物均能造成不利影响。为进一步探究抑食金球藻对浮游动物的影响, 本文以日本虎斑猛水蚤(Tigriopus japonicus)为实验生物, 研究抑食金球藻对日本虎斑猛水蚤摄食、存活、生长发育以及繁殖的影响。日本虎斑猛水蚤具有易于在实验室培养、生长周期短、雌雄异体等优点, 是海洋毒性污染物检测的模式生物。实验利用高效液相色谱分析方法, 在日本虎斑猛水蚤体内色素中检测到了抑食金球藻的特征色素19’-丁酰氧基岩藻黄素(But-fuco), 表明日本虎斑猛水蚤能够摄食抑食金球藻。当微藻生物量(相对碳含量)分别同为0.7 μg/mL和7.2 μg/mL时, 在抑食金球藻中无节幼体发育至桡足幼体及成体的存活率均高于以青岛大扁藻为饵料的对照组, 但无显著差异, 并且在前者中无节幼体的发育时间显著低于后者, 12 d内雌体的产卵次数与产卵量显著高于后者。结果表明, 日本虎斑猛水蚤在抑食金球藻中能进行正常的生命活动, 并且是首次报导的一种能够在抑食金球藻中正常摄食、生存、生长发育和繁殖的浮游动物。因此, 当褐潮发生时, 由于贝类幼体等生物会受到显著的不利影响, 日本虎斑猛水蚤等抗性较强的生物可能会成为优势种, 从而会影响浮游动物群落结构的组成, 进而可能会使整个海洋生态系统发生变化。本研究有助于全面了解褐潮对海洋生态系统的影响。
关键词:  日本虎斑猛水蚤  抑食金球藻  摄食  存活  生长发育  繁殖
DOI:10.11759/hykx20231220001
分类号:X55
基金项目:科技基础资源调查专项(2018YF100200); 中国科学院A类战略性先导科技专项(XDA23050302)
Experimental study on survival, growth, and reproduction of Tigriopus japonicus in Aureococcus anophagefferens-rich environments
ZHOU Huixin1,2, WANG Jinxiu1, WANG Xin1,2, XU Zijun3, YAN Tian1
1.CAS Key Laboratory of Marine Ecology and Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266404, China;2.University of Chinese Academy of Sciences, Beijing 100049, China;3.North China Sea Environmental Monitoring Center, State Oceanic Administration, Qingdao 266033, China
Abstract:
Aureococcus anophagefferens can cause brown tides and adversely affect numerous organisms, such as shellfish and zooplankton. To elucidate the influence of A. anophagefferens on zooplankton, this study used Tigriopus japonicus to evaluate the effects of A. anophagefferens on T. japonicus feeding, survival, growth, and reproduction. T. japonicus has several advantages, such as being easy to cultivate in the laboratory, short growth cycle, and sexual dimorphism; moreover, it is a marine model organism widely used to detect toxic pollutants. Herein, T. japonicus was used as the experimental organism, and Platymonas helgolandica was used as the control. The experiment used high-efficiency liquid chromatographic analysis methods and detected 19’-butyroxyfucoxanthin (But-fuco), a characteristic pigment of A. anophagefferens, in the internal pigment of T. japonicus, indicating that T. japonicus could ingest A. anophagefferens. When the algal biomass was 0.7 μg/mL and 7.2 μg/mL, the survival rate of nauplii to copepod and adults in A. anophagefferens-rich environmentswas higher than that in P. helgolandica-rich environments; however, no significant difference was observed. Moreover, the development time of nauplii in A. anophagefferens-rich environments was lower than that in P. helgolandica-rich environments, and the number of eggs laid by females within 12 days was significantly higher in the former than those in the latter. Results revealed that T. japonicus could perform normal activities in A. anophagefferens-rich environments, reporting for the first time that zooplankton can normally feed, survive, develop, and reproduce in A. anophagefferens-rich environments. Therefore, when brown tides occur, more resistant organisms such as T. japonicus may become the dominant species considering the substantially adverse effects on other organisms such as shellfish larvae, which may affect the composition of the zooplankton community, ultimately affecting the entire marine ecosystem. Thus, this study contributes to a comprehensive understanding of the impact of brown tide on ecosystems.
Key words:  Tigriopus japonicus  Aureococcus anophagefferens  feeding  survive  growth  reproduction
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