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引用本文:张子阳,成永旭,李凤辉,陈四清,常青,陈莹,徐荣静,彭立成,吴丹,边力.盐度对绿鳍马面鲀存活、抗氧化酶及组织结构的影响[J].海洋科学,2024,48(3):85-94.
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盐度对绿鳍马面鲀存活、抗氧化酶及组织结构的影响
张子阳1,2, 成永旭1, 李凤辉2, 陈四清2, 常青2, 陈莹2, 徐荣静3, 彭立成4, 吴丹2, 边力2
1.上海海洋大学水产与生命学院, 上海 200120;2.中国水产科学研究院黄海水产研究所 海水养殖生物育种与可持续产出全国重点实验室, 山东 青岛 266071;3.烟台开发区天源水产有限公司, 山东 烟台 264001;4.宁德市南海水产科技有限公司, 福建 宁德 352102
摘要:
绿鳍马面鲀(Thamnaconus septentrionalis)作为中国沿海新兴网箱养殖种类, 在夏季多雨季节会经历盐度剧烈变化的过程, 因而探明盐度对绿鳍马面鲀存活和生理指标的影响, 对开展网箱养殖具有重要意义。本研究首先测定了绿鳍马面鲀的96 h半致死盐度, 随后分别设置低盐组(15)和高盐组(40), 分析盐度胁迫不同时间后生理指标的动态变化规律, 观察肝脏、鳃和肾脏的组织结构改变。结果显示, 绿鳍马面鲀96 h半致死低盐度为10.74, 半致死高盐度为42.95。低盐组和高盐组鳃Na+-K+-ATP酶活呈上升趋势, 且高于对照组, 在96 h时各组差异性显著(P<0.05)。低盐组和高盐组肝脏超氧化物歧化酶(SOD)和过氧化氢酶(CAT)活性均表现为先上升、后下降的趋势, 在96 h时均低于对照组; 谷胱甘肽过氧化物酶(GSH-PX)活性呈先下降后上升的趋势, 在96 h时低盐组显著低于高盐组(P<0.05)。低盐组和高盐组肝脏丙二醛(MDA)含量在96 h达到最大值, 且显著高于对照组(P<0.05)。组织切片显示, 低盐组和高盐组肝细胞出现空泡和细胞核固缩的现象, 且高盐组更为严重; 鳃丝上皮细胞排列紊乱、细胞坏死, 低盐组鳃丝变宽, 鳃小片变长, 高盐组鳃丝萎缩, 鳃小片间距变大; 肾组织低盐组出现肾小管管腔扩张、肾小囊腔膨大, 高盐组出现肾小管和肾小球坏死的现象。本研究探究了盐度对存活、抗氧化酶及组织结构的影响, 可为绿鳍马面鲀健康养殖提供理论支持。
关键词:  绿鳍马面鲀(Thamnaconus septentrionalis)  盐度  抗氧化  组织结构
DOI:10.11759/hykx20230926001
分类号:S917.4
基金项目:中国水产科学研究院黄海水产研究所基本科研业务费项目(20603022022014); 福建省海洋渔业资源与生态环境重点实验室开放基金课题项目(Z822286); 烟台市科技创新发展计划项目(2022XCZX078); 福建省区域发展项目(2022N3015)
Effect of salinity on survival, antioxidant enzymes and tissue structure of Thamnaconus septentrionalis
ZHANG Ziyang1,2, CHENG Yongxu1, LI Fenghui2, CHEN Siqing2, CHANG Qing2, CHEN Ying2, XU Rongjing3, PENG Licheng4, WU Dan2, BIAN Li2
1.School of Aquatic Sciences and Life Sciences, Shanghai Ocean University, Shanghai 200120, China;2.State Key Laboratory of Mariculture Biobreeding and Sustainable Goods, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China;3.Yantai Development Zone Tianyuan Fishery Co, Yantai 264001, China;4.Ningde Nanhai Aquatic Technology Co, Ningde 352102, China
Abstract:
The greenfin horse-faced filefish (Thamnaconus septentrionalis), an emerging species for cage aquaculture along the coast of China, undergoes significant salinity fluctuations during the rainy season in summer. Therefore, understanding the impact of salinity on the survival and physiological indicators of T. septentrionalis is of great significance for the development of cage aquaculture. In this study, the 96 h semilethal salinity of T. septentrionalis was determined. Subsequently, the low-salinity (15) and high-salinity (40) groups were established. Dynamic changes in physiological indicators after salinity stress at different time points were analyzed, and the changes in the tissue structure of the liver, gills, and kidneys were recorded. The results indicated that the 96-h semilethal salinity was 10.74 for the low-salinity group and 42.95 for the high-salinity group. The Na+-K+-ATPase activity in the gills of both the low- and high-salinity groups indicated an increasing trend, which was significantly higher than that in the control group at 96 h (P.< 0.05). The activities of superoxide dismutase and catalase in the liver of both the low- and high-salinity groups revealed an initial increase followed by a decreasing trend, and at 96 h, the activities were lower in these groups than in the control group. The activity of glutathione peroxidase in the low-salinity group showed a decreasing trend, followed by an increase, and, at 96 h, it was significantly lower than that in the high-salinity group (P< 0.05). Malondialdehyde content in the liver of the low- and high-salinity groups reached a maximum at 96 h, which was significantly higher than that in the control group (P < 0.05). The tissue sections revealed that hepatocytes in the low- and high-salinity groups exhibited vacuolation and nuclear condensation, with more severe effects in the high-salinity group. The gill epithelial cells displayed a disorderly arrangement and cell necrosis, with widened filaments and elongated lamellae in the low-salinity group, whereas the high-salinity group exhibited filament shrinkage and increased lamellar spacing. In the kidney tissues, the low-salinity group showed dilation of the renal tubules and enlargement of the renal sacs, whereas the high-salinity group exhibited necrosis of the renal tubules and glomeruli. The findings on the effects of salinity on survival, antioxidant enzymes, and tissue structure in this study provide theoretical support for the healthy aquaculture of T. septentrionalis.
Key words:  Thamnaconus septentrionalis  salinity  antioxidant  organizational structure
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