| 引用本文: | 牛建峰,王旭雷,郇丽,张宝玉,郑阵兵,解修俊,王立军,顾文辉,王广策.中国紫杉状海门冬配子体和四分孢子体适宜温度和光照强度比较研究[J].海洋科学,2024,48(10):13-21. |
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| 中国紫杉状海门冬配子体和四分孢子体适宜温度和光照强度比较研究 |
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牛建峰1,2, 王旭雷1,2, 郇丽1,2, 张宝玉1,2, 郑阵兵1,2, 解修俊1,2, 王立军1,2, 顾文辉1,2, 王广策1,3,2
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1.水产品种创制与高效养殖重点实验室(中国科学院), 山东 青岛 266404;2.山东省实验海洋生物学重点实验室, 山东 青岛 266404;3.青岛海洋科技中心海洋生物学与生物技术功能实验室, 山东 青岛 266237
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| 摘要: |
| 海门冬(Asparagopsis)作为一种反刍动物甲烷释放的有效抑制物,其生物量获取是促进相关应用领域持续发展的关键,因而海门冬人工栽培日益受到关注。本研究选取中国地区特有紫杉状海门冬配子体与四分孢子体为研究对象,光响应曲线测定结果显示配子体半饱合光强约为56 μmol/(m2·s),四分孢子体的约为88 μmol/(m2·s)。光照强度对藻体光合作用及呼吸作用的影响结果显示,四分孢子体光合活性显著高于配子体,且具有较高的强光耐受能力。配子体和四分孢子体的光补偿点均约为10 μmol/(m2·s),四分孢子体饱和光强在250~270 μmol/(m2·s),相较于配子体的100 μmol/(m2·s),显示出了更宽广的光强适应能力。在15℃~31℃温度范围内,四分孢子体和配子体的光合作用系统均未受到显著影响,而四分孢子体有效量子产额在长时间29℃条件下轻微下降。研究结果还显示,两种藻体在可溶性总蛋白、藻红蛋白及叶绿素a含量上未见显著差异,丝状四分孢子体因具有更大的比表面积,表现出显著高于配子体的光合效率。同时紫杉状海门冬四分孢子体具有较广的光强适应范围,因而是一种更适于基于反应器的规模化培养的藻体形态。 |
| 关键词: 紫杉状海门冬(Asparagopsis taxiformis) 四分孢子体 配子体 温度 光照强度 |
| DOI：10.11759/hykx20240709001 |
| 分类号:Q539;Q51 |
| 基金项目:国家重点研发计划资助项目(2022YFF0802201);山东省重点研发计划重大科技创新项目(2022LZGC004);国家自然科学基金资助项目(42376091);山东省泰山学者项目研究基金(tspd20210316);财政部和农业农村部:国家现代农业产业技术体系资助项目(CARS-50) |
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| A comparative study on the temperature and light intensity suitable for gametophytic and tetrasporophytic growth of ChineseAsparagopsis taxiformis |
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NIU Jianfeng1,2, WANG Xülei1,2, HUAN Li1,2, ZHANG Baoyu1,2, ZHENG Zhenbing1,2, XIE Xiujun1,2, WANG Lijun1,2, GU Wenhui1,2, WANG Guangce1,3,2
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1.Key Laboratory of Breeding Biotechnology and Sustainable Aquaculture (CAS), Qingdao 266404, China;2.Shandong Province Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266404, China;3.Laboratory for Marine Biology and Biotechnology, Qingdao Marine Science and Technology Center, Qingdao 266237, China
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| Abstract: |
| Research on the artificial cultivation of Asparagopsis has received increasing attention owing to its potential environmental benefits as an effective methane-release inhibitor for ruminants. Here, the light response curves of the gametophytes and tetrasporophytes of Asparagopsis taxiformis were determined first at the onset of light saturations (Ik) of 56 and 88 μmol/(m·s), respectively. Then, different light conditions were set up to study the effects of irradiation on the photosynthesis and respiration rates in the algae. The results showed that the tetrasporophytes exhibited a higher photosynthetic activity in comparison to gametophytes. The light compensation point, established using a liquid-phase oxygen electrode, was consistently identified at 10 μmol/(m·s) in both the gametophytes and tetrasporophytes. Notably, the light intensity saturation point for the tetrasporophytes was 250–270 μmol/(m·s), substantially surpassing that of the gametophytes at 100 μmol/(m·s). However, the soluble total proteins, phycoerythrin, and chlorophyll-a contents did not differ significantly between the two generations. Within 15℃–31℃, the photosynthetic systems of both the tetrasporophytes and gametophytes did not exhibit any stress-related damage, while the effective quantum yield of the tetrasporophytes was slightly affected at >29℃ during the fifth day of cultivation. These results also revealed no marked variations in the soluble total protein, phycoerythrin, and chlorophyll-a levels between the two algal morphologies. The filamentous tetrasporophyte exhibited remarkably higher photosynthetic efficiency than the gametophyte due to its larger surface area. Therefore, the tetrasporophyte of A. taxiformis is a prime candidate for future reactor-based large-scale commercial cultivation compared to the gametophyte due to its wide adaptability to varying light intensities and higher photosynthetic activity. |
| Key words: Asparagopsis taxiformis tetrasporophyte gametophyte temperature light intensity |
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