| 引用本文: | 刘涛,安明明,裴炎沼,秦梓凌,王淑敏,周斌.石油烃对鳗草(Zostera marina)幼苗光合作用的毒性效应研究[J].海洋科学,2025,49(3):40-50. |
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| 石油烃对鳗草(Zostera marina)幼苗光合作用的毒性效应研究 |
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刘涛1, 安明明1, 裴炎沼2, 秦梓凌2, 王淑敏2, 周斌2
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1.中海油研究总院有限责任公司, 北京 100027;2.中国海洋大学 海洋生命学院, 山东 青岛 266003
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| 摘要: |
| 为评估石油烃对鳗草幼苗光合作用的影响, 设置0、2.5、5、10、20、40 mg/L 0号柴油水溶液(water-accommodated fraction, WAF)胁迫培养8 d, 研究鳗草幼苗光合色素含量(叶绿素a、b)、最大光化学量子产量(Fv/Fm)、荧光淬灭参数(NPQ、qL)以及光合电子传递速率的变化; 并结合0 (对照组)、5、10、20、40 mg/L处理组转录组分析, 观察光合系统差异表达通路, 探讨了石油烃胁迫下鳗草幼苗光合系统的毒性效应和机制。结果表明: 在8 d的实验周期内, 鳗草幼苗叶绿素a、叶绿素b含量以及Fv/Fm、qL和rETR等叶绿素荧光参数与石油烃浓度呈负相关, 在40 mg/L处理组中较对照组分别降低12.47%、16.65%、6.01%、59.82%和58.12%; 鳗草幼苗NPQ参数随浓度增加,呈现先升高后降低的趋势, 在10 mg/L处理组达到最大,表明随着石油烃浓度升高,鳗草幼苗光合活性和光保护能力降低, 光合系统被显著抑制; 转录组分析结果显示与对照组相比, 5、10 mg/L处理组差异表达基因较多, 分别为4 272和3 254个。5 mg/L处理组差异表达基因上调最多达3 219个, 20 mg/L处理组差异性表达基因下调最多达1 135个。胁迫期间多种与植物光合作用和生长相关基因表达受到影响, 如GIL1、ELIP1和FLU上调, SRR1和WEL2下调, 表明在高浓度石油烃胁迫下, 鳗草幼苗光合作用被抑制, 与光合色素以及叶绿素荧光参数变化一致。 |
| 关键词: 石油烃 鳗草幼苗 光合系统 荧光参数 |
| DOI：10.11759/hykx20231022001 |
| 分类号:Q945.78 |
| 基金项目:国家自然科学基金（42076147） |
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| Toxicity and mechanisms of water-accommodated fractions on photosynthesis in Zostera marina |
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LIU Tao1, AN Mingming1, PEI Yanzhao2, QIN Ziling2, WANG Shumin2, ZHOU Bin2
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1.China National Offshore Oil Corporation, Beijing 100027, China;2.College of Marine Life Science, Ocean University of China, Qingdao 266003, China
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| Abstract: |
| To evaluate the effects of water-accommodated fractions (WAFs) on photosynthesis in Zostera marina seedlings, 0, 2.5, 5, 10, 20, and 40 mg/L WAFs were applied for 8 days. The changes in photosynthetic pigment content (chlorophyll a and b), maximum photochemical quantum yield (Fv/Fm), fluorescence quenching parameters (QNP and qL), and photosynthetic electron transfer rate of the seedlings were studied. It was combined with the transcriptomics of 0 (Group CG), 5 (Group A), 10 (Group B), 20 (Group C), and 40 mg/L (Group D) treatment groups to observe the differential expression of genes associated with the photosynthetic pathways. The toxic effects on the photosynthetic system and underlying mechanisms under WAF stress were explored. The results showed that during the 8-day experimental period, a WAF concentration of less than 5 mg/L did not significantly impact chlorophyll a, chlorophyll b, and Fv/Fm in the seedlings. At concentrations greater than 5 mg/L, these parameters were markedly reduced by 12.47%, 16.65%, and 6.01%, respectively in the 40 mg/L group. A WAF concentration of less than 10 mg/L had no remarkable effects on the qL and rETR. However, at concentrations greater than 10 mg/L, these parameters were significantly suppressed by 59.82% and 58.12% in the 40 mg/L group. At a WAF concentration of greater than 2.5 mg/L, the QNP increased markedly by 6.6%, 9.03%, 13.11%, 11.92%, and 10.72%, respectively, in each treatment group on the 8th day. These findings indicate that low concentrations (2.5 and 5 mg/L) of WAF had a relatively small impact on the photosynthetic system of Z. marina seedlings; however, the photosynthetic system was damaged under medium-high concentrations (10, 20, and 40 mg/L). Transcriptomics indicated that the number of differentially expressed genes in Groups B and C were 4 272 and 3 254 genes, respectively, compared to Group CG. The maximum number of differentially upregulated genes in Group B was 3 219, and that of downregulated genes in Group D was 1 135. Transcriptomics revealed that the photosynthesis pathway was significantly affected by WAF stress. |
| Key words: water-accommodated fraction (WAF) Zostera marina seedlings photosynthesis chlorophyll fluorescence |
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