| 引用本文: | 陈宇欣,王映夏,陈天颖,庄昀筠,李爱峰,陈洪举,刘光兴.珠江口藻毒素检出水域小型真核浮游生物及其共附生细菌群落特征分析[J].海洋科学,2025,49(10):52-63. |
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| 珠江口藻毒素检出水域小型真核浮游生物及其共附生细菌群落特征分析 |
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陈宇欣1,2, 王映夏1,2, 陈天颖1,2, 庄昀筠1,2,3, 李爱峰1,2,3, 陈洪举1,2,3, 刘光兴1,2,3
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1.中国海洋大学环境科学与工程学院, 山东 青岛 266100;2.中国海洋大学海洋环境与生态教育部重点实验室, 山东 青岛 266100;3.青岛海洋科技中心海洋生态与环境科学功能实验室, 山东 青岛 266237
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| 摘要: |
| 有毒有害藻对海洋生态系统稳定与公共健康构成威胁, 但其对浮游生物群落多样性及种间互作的影响尚不明确。本研究于2019年6月在珠江口水域开展, 针对存在有毒有害藻且检出扇贝毒素(pectenotoxins-2, PTX2)和类虾夷扇贝毒素(homo-yessotoxin, hYTX)的典型站位,采集小型浮游生物(> 20 μm)并通过DNA宏条形码技术,解析了小型真核浮游生物及其共附生细菌的群落多样性、构建机制与共现网络。结果显示, 有毒有害藻丰度显著影响了群落结构: 真核浮游生物及其共附生细菌均可划分为有毒有害藻高丰度区群落(H组)和低丰度区群落(L组)两类, 但两组间α多样性无显著差异。小型真核浮游生物群落以硅藻(18.73%)和甲藻(16.58%)为主, 共附生细菌群落以γ-变形菌(73.79%)和α-变形菌(13.76%)为主。温度和跨域生物相互作用共同影响了真核浮游生物及其共附生原核生物的群落结构; 其中, 真核浮游生物群落还受到盐度和hYTX浓度的影响, 共附生细菌群落则受到叶绿素a和溶解氧浓度的影响, 共附生细菌群落对环境过滤作用表现出更高的敏感性。随机过程(扩散限制和生态漂移)主导群落构建。在共现网络中, 硅藻、甲藻、寄生甲藻和变形菌之间存在广泛共现关系, 占共现网络总边数的29.50%; 超过77.32%的节点和76.78%的关键OTU均来自非丰富类群; 关键OTU相连的边中, 34.8%为真核生物-细菌间的跨域互作边。本研究揭示了有毒有害藻对小型真核浮游生物及其共附生细菌群落结构的影响, 并为进一步了解非丰富类群的生态功能奠定基础。 |
| 关键词: 有害藻 珠江口海域 构建机制 共现网络 微生物相互作用 |
| DOI：10.11759/hykx20250430003 |
| 分类号:P735 |
| 基金项目:国家自然科学基金(42276134); 科技基础资源调查专项(2018FY10020001) |
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| Characterization of the microeukaryotic plankton community and associated bacteria in the phycotoxin-contaminated waters of the Pearl River Estuary |
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CHEN Yuxin1,2, WANG Yingxia1,2, CHEN Tianying1,2, ZHUANG Yunyun1,2,3, LI Aifeng1,2,3, CHEN Hongju1,2,3, LIU Guangxing1,2,3
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1.College of Environmental Science and Engineering, Ocean University of China, Qingdao 266100, China;2.Key Laboratory of Marine Environment and Ecology, Ministry of Education, Ocean University of China, Qingdao 266100, China;3.Laboratory for Marine Ecology and Environmental Science, Qindao Marine Science and Techonology Center, Qindao 266237, China
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| Abstract: |
| Harmful algae significantly threaten marine ecosystem stability and public health. However, their impact on planktonic community diversity and interspecific interactions remains unclear. In June 2019, microplanktons (>20 μm in size) were sampled from the Pearl River Estuary, where harmful algae as well as the phycotoxins pectenotoxin-2 (PTX2) and homo-yessotoxin (hYTX) were detected. DNA metabarcoding was employed to analyze the diversity, assembly mechanisms, and co-occurrence networks of microeukaryotic plankton and associated bacterial communities. Results showed that both communities were mainly shaped by the abundance of toxic/harmful algae. These were divided into two clusters: high and low abundance (H and L groups, respectively). However, the α-diversity did not show any significant intergroup differences. The plankton community was dominated by Bacillariophyta (18.73%) and Dinophyceae (16.58%), while the bacterial community was dominated by γ- (73.79%) and α- (13.76%) Proteobacteria. Temperature and cross-domain biotic interactions co-shaped the plankton community. It was additionally influenced by salinity and hYTX concentration. However, the bacterial communities responded to chlorophyll a and dissolved O2,while exhibiting an enhanced sensitivity to environmental filtering. Stochastic processes (dispersal limitation and ecological drift) governed community assembly. Co-occurrence networks were dominated by the associations among Bacillariophyta, Dinophyceae, Syndiniales, and Proteobacteria, accounting for 29.5% of the total edges. Non-abundant taxa constituted 77.32% of the nodes and 76.78% of the keystone OTUs, while 34.8% of the edges linked to these OTUs were represented by eukaryote-bacteria associations. In summary, this study elucidates the impacts of toxic algae on the community structure of microeukaryotic plankton and associated bacteria, providing a foundation for further research into the ecological roles of non-dominant taxa. |
| Key words: harmful algae Pearl River Estuary assembly mechanism co-occurrence network microbial interactions |
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