| 引用本文: | 周胜杰,马振华,唐贤明,吴洽儿,胡静,杨蕊,戴世明.波纹唇鱼♀×鞍带石斑鱼♂杂交子一代胚胎发育[J].海洋科学,2025,49(2):47-55. |
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| 波纹唇鱼♀×鞍带石斑鱼♂杂交子一代胚胎发育 |
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周胜杰1,2,3,4,5, 马振华1,2,3,4,5, 唐贤明6, 吴洽儿3, 胡静1,2,3,4,5, 杨蕊1,2,4,5, 戴世明1,2,3,4,5
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1.三亚热带水产研究院, 海南 三亚 572018;2.海南省深远海渔业资源高效利用与加工重点实验室, 海南 三亚 572018;3.中国水产科学研究院南海水产研究所, 广东 广州 510300;4.深远海养殖与加工海南省工程研究中心, 海南 三亚 572018;5.南海渔业资源保护与应用国际联合研究中心, 海南 三亚 572018;6.海南省热带海水养殖技术重点实验室, 海南 海口 570100
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| 摘要: |
| 本试验旨在通过人工挤取波纹唇鱼(Cheilinus undulatus)♀的卵和鞍带石斑鱼(Epinephelus lanceolatus)♂的精液,并进行人工混合受精,以观察和描述杂交受精卵胚胎的发育过程及其特征。采用光学显微镜观察并记录各个发育阶段的特征。研究发现,在水温为27.5℃± 0.5℃的条件下,波纹唇鱼♀×鞍带石斑鱼♂受精卵直径为(574 ± 31)μm;历时26 h 21 min开始孵化出膜;波纹唇鱼♀×鞍带石斑鱼♂与其他硬骨鱼类胚胎发育特征基本相同,胚胎发育过程可分为受精卵阶段、卵裂阶段、囊胚阶段、原肠胚阶段、神经胚阶段、器官形成阶段和孵化出膜阶段,共7个阶段;波纹唇鱼♀×鞍带石斑鱼♂子一代原肠胚的中晚期与神经胚的早中期高度重合,容易混淆无法区分。研究结果表明,波纹唇鱼♀×鞍带石斑鱼♂子一代胚胎发育时长较长,与直径大小相关的发育时长规律出现偏离,属于特例情况;波纹唇鱼♀×鞍带石斑鱼♂子一代胚胎的发育时长长于父本和母本,显示受到杂交的影响而延长;更接近于父本,表明杂交子一代胚胎发育速度受父本影响较大。 |
| 关键词: 波纹唇鱼(Cheilinus undulatus) 鞍带石斑鱼(Epinephelus lanceolatus) 受精卵 胚胎发育 杂交 |
| DOI：10.11759/hykx20240814002 |
| 分类号:S961.5 |
| 基金项目:三亚崖州湾科技城科技专项(SCKJ-JYRC-2023-42);海南省重大科技计划项目(ZDK2021011);海南省热带海水养殖技术重点实验室开放课题基金资助项目(HNTMTOF202303) |
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| Development of female humphead wrasse♀ × male giant grouper♂ hybrid embryos |
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ZHOU Shengjie1,2,3,4,5, MA Zhenhua1,2,3,4,5, TANG Xianming6, WU Qiaer3, HU Jing1,2,3,4,5, YANG Rui1,2,4,5, DAI Shiming1,2,3,4,5
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1.Sanya Tropical Fisheries Research Institute, Sanya 572018, China;2.Key Laboratory of Efficient Utilization and Processing of Marine Fishery Resources of Hainan Province, Sanya 572018, China;3.South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, China;4.Hainan Engineering Research Center for Deep-sea Aquaculture and Processing, Sanya 572018, China;5.International Joint Research Center for Conservation and Application of Fishery Resources in the South China Sea, Sanya 572018, China;6.Hainan Provincial Key Laboratory of Tropical Maricultural Technologies, Haikou 570100, China
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| Abstract: |
| This experiment aimed to artificially extract eggs from a female humphead wrasse (Cheilinus undulatus) and the sperm from a male giant grouper (Epinephelus lanceolatus), conduct artificial fertilization, and observe as well as describe the development process and characteristics of the hybrid embryos. The characteristics of each developmental stage were observed and recorded using an optical microscope. The study found that at a water temperature of 27.5℃ ± 0.5℃, the diameter of the fertilized eggs was 574 ± 31 μm; hatching took 26 h and 21 min to start. The embryonic developmental characteristics of the hybrid embryos were the same as those of other bony fish species. The embryonic development process was divided into seven stages: fertilized egg, cleavage, blastula, gastrula, neurula, organ formation, and hatching membrane. The mid-late stage of the original intestine embryo overlapped significantly with the early-middle stage of the neural embryo, causing confusion and making it difficult to distinguish. These study results indicate that the developmental time of the hybrid embryos was longer, deviating from the normal developmental time pattern related to diameter and size, which is unique. The developmental time of the hybrid embryos was longer than those of the parent species, indicating an extension influenced by hybridization. It is more similar to that of the male parent, suggesting a marked influence of the male parent on the developmental speed. |
| Key words: Cheilinus undulatus Epinephelus lanceolatus artificial fertilization embryo development hybridization |
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