| 摘要: |
| 为揭示生物合成FeS (Bio-FeS)对海洋硫酸盐还原菌Desulfovibrio bizertensis SY-1胞外电子传递(EET)的调控机制,对加入20 mg/L、40 mg/L、60 mg/L、80 mg/L及100 mg/L Bio-FeS的Desulfobacterales bizertensis SY-1体系通过计时电流法(I-t)、电化学阻抗谱(EIS)、差分脉冲伏安法(DPV)及循环伏安法(CV)等方法进行分析测试。结果表明,加入Bio-FeS后生物电流明显增强,Bio-FeS促进了D.bizertensis SY-1细胞色素c的合成进而增强氧化还原活性,同时也增强了电极界面电荷存储能力,降低了体系内电荷转移电阻及溶液电阻,提高了D.bizertensis SY-1的胞外电子传递效率。这一研究为海洋工程中微生物腐蚀防护及微生物燃料电池(MFCs)的电子介体设计提供了新思路。 |
| 关键词: 硫酸盐还原菌 胞外电子传递 计时电流法 脉冲伏安法 循环伏安法 |
| DOI：10.11759/hykx20250315001 |
| 分类号:TL271+.3 |
| 基金项目:国家自然科学基金 (42076044; 42206126) |
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| Study on the accelerating extracellular electron transfer in marine sulfate-reducing bacterium Desulfovibrio bizertensis SY-1 by biosynthesis of FeS |
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YANG Jinfeng1,2, WANG Yanan1, DUAN Jizhou1
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1.Key Laboratory of Marine Key Materials, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266000, China;2.University of Chinese Academy of Sciences, Beijing 100049, China
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| Abstract: |
| In order to reveal the regulation mechanism of biosynthesized FeS (Bio-FeS) on the extracellular electron transfer (EET) of the marine sulfate-reducing bacterium Desulfovibrio bizertensis SY-1(D. bizertensis SY-1), the addition of 20 mg/L, 40 mg/L, 60 mg/L, 80 mg/L, and 100 mg/L Bio-FeS to the D. bizertensis SY-1 system with 20 mg/L, 40 mg/L, 60 mg/L, 80 mg/L and 100 mg/L of Bio-FeS was analyzed and tested by chronoamperometry (I-t), electrochemical impedance spectroscopy (EIS), differential pulse voltammetry (DPV) and cyclic voltammetry (CV). The results showed that the bioelectrical current was significantly enhanced by the addition of Bio-FeS, and Bio-FeS promoted the synthesis of D. bizertensis SY-1 cytochrome c and thus enhanced the redox activity, and also enhanced the electrode interfacial charge storage capacity, lowered the intra-system charge transfer resistance and solution resistance, and increased the EET efficiency of D.bizertensis SY-1. electron transfer efficiency. This study provides new ideas for microbial corrosion protection in marine engineering and the design of electron mediators for microbial fuel cells (MFCs). |
| Key words: sulfate-reducing bacteria extracellular electron transfer I-t DPV CV |
