| 引用本文: | 王昕,刘畅,葛香云,邢荣娥,刘松,李克成.栉孔扇贝内脏多糖的提取分离与生物活性研究[J].海洋科学,2025,49(6):98-111. |
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| 栉孔扇贝内脏多糖的提取分离与生物活性研究 |
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王昕1,2, 刘畅1,3, 葛香云1, 邢荣娥1,2, 刘松1,2, 李克成1,2
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1.中国科学院海洋研究所, 山东 青岛 266000;2.青岛海洋科技中心 海洋药物与生物制品功能实验室, 山东 青岛 266000;3.青岛农业大学, 山东 青岛 266237
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| 摘要: |
| 以栉孔扇贝内脏副产物为原料,对其中多糖的提取、分离及活性进行了研究。首先选用酶解法制备粗多糖,通过单因素实验筛选,发现最佳酶解条件为中性蛋白酶与胰蛋白酶的比例为1∶2,酶解反应pH=7,反应温度40℃,酶解时间2 h。将粗多糖经DEAE-52离子交换柱和Sephadex G-100凝胶排阻色谱柱进行分离纯化,得到一种分子量均一的氨基多糖CFP4-G3,其分子量为4 935 Da,糖醛酸质量分数为4.081%,硫酸根质量分数为4.335%。红外光谱和紫外光谱分析证实了CFP4-G3符合氨基多糖的典型结构特征。生物学功能研究结果表明所得多糖CFP4-G3具有显著的抗氧化活性,能有效清除羟自由基、超氧阴离子以及DPPH,且具有较强的还原力;CFP4-G3还有良好的免疫调节活性,能够显著刺激小鼠巨噬细胞NO的释放和TNF-α与IL-6的分泌。以上研究结果为扇贝内脏多糖的深度开发奠定重要理论基础。 |
| 关键词: 栉孔扇贝 内脏 多糖 分离纯化 生物活性 |
| DOI：10.11759/hykx20250224001 |
| 分类号:Q539 |
| 基金项目:国家重点研发计划专项课题(2023YFD2401105); 现代农业产业技术体系专项(CARS-49) |
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| Extraction, isolation, and bioactivity of visceral polysaccharides from Chlamys farreri |
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WANG Xin1,2, LIU Chang1,3, GE Xiangyun1, XING Ronge1,2, LIU Song1,2, LI Kecheng1,2
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1.Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266000, China;2.Laboratory for Marine Drugs and Bioproducts, Qingdao Marine Science and Technology Center, Qingdao 266000, China;3.Qingdao Agricultural University, Qingdao 266237, China
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| Abstract: |
| In this study, we examined the extraction, separation, and activity of polysaccharides from Chlamys farreri viscera byproducts. The crude polysaccharides were primarily prepared using the enzyme digestion method. The optimal enzyme digestion conditions were identified using a one-way experiment, which revealed an optimal neutral protease to trypsin ratio of 1∶2 at pH 7, a reaction temperature of 40 ℃, and a digestion time of 2 h. The crude polysaccharides were subjected to separation and purification using a DEAE-52 ion exchange column and a Sephadex G-100 gel exclusion chromatographic column, respectively. The molecular weight of the purified CFP4-G3 fraction was 4 935 Da, with a uronic acid content of 4.081% and a sulfate content of 4.335%. Infrared absorption spectroscopy and ultraviolet spectroscopy confirmed that CFP4-G3 conformed to the typical structural features of amino polysaccharides. Bioactivity assays of the resulting CFP4-G3 polysaccharide demonstrated that it had strong antioxidant activity, including scavenging of hydroxyl radicals, superoxide anion, and DPPH, and also possessed substantial reducing power. In addition, CFP4-G3 exhibited strong immunostimulatory activity. Moreover, CFP4-G3 substantially stimulated NO release and the secretion of TNF-α and IL-6 in RAW264.7 mouse macrophages. The above findings provide a crucial theoretical foundation for the in-depth development of scallop viscera polysaccharides. |
| Key words: Chlamys Farreri visceral polysaccharides isolation and purification bioactivities |
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