| 摘要: |
| 于1997年3月–1997年5月，取山东长岛和青岛太平角海区的虾夷扇贝和栉孔扇贝。采用自然排放法和解剖法取精液，用自然海水作基础溶液，采用不同的抗冻保护剂、冷冻速度、平衡时间，对精子的存活率、受精率和孵化率进行了研究。结果表明，两种扇贝的精子以自然海水为基础溶液，加8％–10％的DMSO作为抗冻保护剂，距液氮表面25cm、20cm、10cm各停留8min，移入液氮中保存；解冻时，距液氮表面10cm、15cm、20cm各停留6min，7–11°C流水解冻，置4°C 20min，精于存活率为80％–90％，受精率为85％–95％，孵化率为70％–85％。 |
| 关键词: 虾夷扇贝 栉孔扇贝 精子 超低温保存 |
| DOI： |
| 分类号: |
| 基金项目:国家海洋“863”计划资助项目，8638190102号 |
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| STUDIES ON SPERMATOZOA CRYOPRESERVATION OF SCALLOPS, PATINOPECTEN YESSONESIS AND CHLAMYS FARRERI |
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YANG Ai-guo1, WAN Qing-yin1, KONG Jie1, LIU Zhi-hong1, LIU Ping1, WANG Ru-cai2
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1.Yellow Sea Fisheries Research Insritute, Chinese Academy of Fishery deiences, Qingdao, 266071;2.College of Fisherier, Ocean
University of Qingdao, Qingdao, 266003
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| Abstract: |
| Spermatozoa cryopreservation of scallops Patinopecten yessonesis and Chlamys farreri from Changdao and Qingdao in Shandong Province was studied in 1997. Spermatozoa were collected from mature scallops by natural spawning and artificial dissecting. A series of tests were conducted to develop a suitable freezing and thawing technique for the cryopreservation of the scallop spermatozoa. The effects of diluent compositions on spermatozoa survival rate and time after thawing showed that natural sea water was the best diluent and the average survival rate of thawed spermatozoa was 80%–90% and the survival time was more than 2h. DMSO was better than glycerin in cryopreservation of spermatozoa of scallops and the spermatozoa survival rate and time were 85%–90% and 2.5–3.5 h respectively. The results of freezing and thawing tests showed that the survival rate of the spermatozoa could be as high as 80%–85% when spermatozoa were maintained at 25 cm, 20 cm or 10 cm above LN2 surface for 8–10 min. the thawing test showed that the highest survival rate of LN2 frozen-thawed spermatozoa could be obtained when the spermatozoa were maintained at 10 cm, 15 cm or 20 cm above LN2 for 15–30 min and then immersed in tap water (7–11°C) till melting. The survival rate and time were 80%–90% and approximately 3 h, respectively. Fresh eggs could be fertilized by cryo-preserved spermatozoa and the fertilization rate and hatchery rate were 85%–95% and 70%–85%, respectively. |
| Key words: Patinopecten yessonesis, Chlamys farreri, Spermatozoa, Cryopreservation |
