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| 褐点石斑鱼脱鳞病病原菌的分离与鉴定 |
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徐先栋1,2, 谢珍玉1,3,4, 欧阳吉隆1, 王世锋1,3,4, 周永灿1,3,4
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1.海南大学 海洋学院;2.江西省水产科学研究所;3.海南大学 海洋生物实验教学示范中心;4.海南大学 海南省热带水生生物技术重点实验室
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| 摘要: |
| 从海南陵水新村港网箱养殖脱鳞病濒死褐点石斑鱼(Epinephelus fuscoguttatus)体内分离到3 株优势菌株XC08061、XC08062 和XC08063, 经回归感染试验确定为致病菌。这3 株细菌经生化鉴定以及16S rDNA 测序分析, 确定均为哈氏弧菌(Vibrio harveyi)。其中, XC08061 对体长为12～14 cm 褐点石斑鱼的半致死剂量为5.8×102 CFU/g 鱼体质量。药敏试验表明, 该菌具有较强的耐药性, 在所检测的25种抗菌药物中, 仅对新生霉素、头孢噻肟、先锋必/舒巴坦、诺氟沙星、依诺沙星和庆大霉素6 种抗菌药物敏感。 |
| 关键词: 褐点石斑鱼(Epinephelus fuscoguttatus) 哈氏弧菌(Vibrio harveyi) 脱鳞病 分离 鉴定 |
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| 基金项目:国家自然科学基金(40866002、30760190); 农业科技成果转化项目(2009GB2E200302); 海南省基金项目:育厅研究生创新课题(Hswsy2009-22); 国家级第二批高等学校特色专业建设点资金(TS10477) |
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| Isolation and identification of bacterial pathogens from Epinephelus fuscoguttatus with scale-peeled syndrome disease |
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| Abstract: |
| A serious scale-peeled disease was broken out in the cage cultured brown-marbled grouper Epinephelus fuscoguttatus (weight 30-35g) in Lingshui County, Hainan province, China. The moribund/dead groupers were characterized by scale-peeled, rupture of gallbladder and ascites. Three strains of bacteria, XC08061、XC08062 and XC08063, were isolated from the liver, spleen and /or the kidney of the diseased grouper and they were all proved to be pathogens by artificial infection. The moribund/dead fish exhibited scale-peeled as that observed in natural outbreak. All these three strains were identified to be Vibrio harveyi by biochemical tests and 16S rDNA sequences analysis. The LD50 value of XC08061 for 12-14 cm E. fuscoguttatus is 5.8×102 colony forming units (CFU)/g of fish body. Drug susceptibility tests with 25 antibiotics indicated that the pathogen was only sensitive to novobiocin, cefotaxime, cefobid/sulbactam, norfloxacin, enoxacin and gentamicin, exhibiting relatively strong drug tolerance. |
| Key words: Epinephelus fuscoguttatus Vibrio harveyi scale-peeled syndrome disease isolation identification |